Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection

S. Loret; G. Nollevaux; R. Remacle; M. Klimek; I. Barakat; P. Deloyer; C. Grandfils; G. Dandrifosse

doi:10.1016/j.jchromb.2004.02.033

Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection

S. Loret, G. Nollevaux, R. Remacle, M. Klimek, I. Barakat, P. Deloyer, C. Grandfils, G. Dandrifosse

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20±9.30% and 0.12±0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52±0.35 expressing a low diffusion of this poorly permeability marker.

langue originale	Anglais
Pages (de - à)	195-202
Nombre de pages	8
journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	805
Numéro de publication	2
Les DOIs	https://doi.org/10.1016/j.jchromb.2004.02.033
Etat de la publication	Publié - 15 juin 2004

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10.1016/j.jchromb.2004.02.033

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Loret, S., Nollevaux, G., Remacle, R., Klimek, M., Barakat, I., Deloyer, P., Grandfils, C., & Dandrifosse, G. (2004). Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 805(2), 195-202. https://doi.org/10.1016/j.jchromb.2004.02.033

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title = "Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection",

abstract = "We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20±9.30% and 0.12±0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52±0.35 expressing a low diffusion of this poorly permeability marker.",

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author = "S. Loret and G. Nollevaux and R. Remacle and M. Klimek and I. Barakat and P. Deloyer and C. Grandfils and G. Dandrifosse",

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Loret, S, Nollevaux, G, Remacle, R, Klimek, M, Barakat, I, Deloyer, P, Grandfils, C & Dandrifosse, G 2004, 'Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, VOL. 805, Numéro 2, p. 195-202. https://doi.org/10.1016/j.jchromb.2004.02.033

Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection. / Loret, S.; Nollevaux, G.; Remacle, R. et al.
Dans: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol 805, Numéro 2, 15.06.2004, p. 195-202.

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

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T1 - Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection

AU - Loret, S.

AU - Nollevaux, G.

AU - Remacle, R.

AU - Klimek, M.

AU - Barakat, I.

AU - Deloyer, P.

AU - Grandfils, C.

AU - Dandrifosse, G.

PY - 2004/6/15

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AB - We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20±9.30% and 0.12±0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52±0.35 expressing a low diffusion of this poorly permeability marker.

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Loret S, Nollevaux G, Remacle R, Klimek M, Barakat I, Deloyer P et al. Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2004 juin 15;805(2):195-202. doi: 10.1016/j.jchromb.2004.02.033

Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection

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