TY - JOUR
T1 - An alternative cold chain for storing and transporting East Coast fever vaccine
AU - Atuhaire, David Kalenzi
AU - Lieberman, Daniel
AU - Marcotty, Tanguy
AU - Musoke, Antony Jim
AU - Madan, Damian
N1 - Funding Information:
This work was supported by the Intellectual Ventures Global Good Fund . The authors thank Dr. Jeremy Salt of Global Alliance for Livestock Veterinary Medicines for constituting an East Coast Fever—Infection and Treatment Method advisory committee that gave valuable guidance of the work. We appreciate the contribution of George Chaka, Namukolo Muyamwa, Osbert Mphukira Pangani, Fiskani Kaula and entire technical team of Centre for Ticks and Tick-Borne Diseases towards the laboratory and animal studies. The authors also thank Toan Huynh and Victoria Hunt for technical assistance and Mark Newell, Zahra Radjavi, Manan Shukla, Sheana Creighton, and Marie Connett for useful discussions.
Publisher Copyright:
© 2020
PY - 2020/12
Y1 - 2020/12
N2 - East Coast fever (ECF) is an often fatal, economically important cattle disease that predominantly affects eastern, central, and southern Africa. ECF is controlled through vaccination by means of simultaneous injection of oxytetracycline and cryogenically preserved stabilate containing live, disease-causing parasites. Storage and transportation of the stabilate requires liquid nitrogen, a commodity that is commonly unreliable in low-resource settings. Here we show that storage of conventionally prepared stabilate at −80 °C for up to 30 days does not significantly affect its ability to infect cultured peripheral blood mononucleated cells or live cattle, suggesting an alternative cold chain that maintains these temperatures could be used to effectively manage ECF.
AB - East Coast fever (ECF) is an often fatal, economically important cattle disease that predominantly affects eastern, central, and southern Africa. ECF is controlled through vaccination by means of simultaneous injection of oxytetracycline and cryogenically preserved stabilate containing live, disease-causing parasites. Storage and transportation of the stabilate requires liquid nitrogen, a commodity that is commonly unreliable in low-resource settings. Here we show that storage of conventionally prepared stabilate at −80 °C for up to 30 days does not significantly affect its ability to infect cultured peripheral blood mononucleated cells or live cattle, suggesting an alternative cold chain that maintains these temperatures could be used to effectively manage ECF.
KW - Cryopreservation
KW - Dry ice
KW - East Coast Fever
KW - ECF
KW - Muguga cocktail vaccine
KW - Theileria parva
UR - http://www.scopus.com/inward/record.url?scp=85095757233&partnerID=8YFLogxK
U2 - 10.1016/j.vetpar.2020.109304
DO - 10.1016/j.vetpar.2020.109304
M3 - Article
C2 - 33161281
AN - SCOPUS:85095757233
SN - 0304-4017
VL - 288
JO - Veterinary Parasitology
JF - Veterinary Parasitology
M1 - 109304
ER -
