Advances in ultra-high performance liquid chromatography coupled to tandem mass spectrometry for sensitive detection of several food allergens in complex and processed foodstuffs

Mélanie Planque, Thierry Arnould, Marc Dieu, Philippe Delahaut, Patricia Renard, Nathalie Gillard

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

Sensitive detection of food allergens is affected by food processing and foodstuff complexity. It is therefore a challenge to detect cross-contamination in food production that could endanger an allergic customer's life. Here we used ultra-high performance liquid chromatography coupled to tandem mass spectrometry for simultaneous detection of traces of milk (casein, whey protein), egg (yolk, white), soybean, and peanut allergens in different complex and/or heat-processed foodstuffs. The method is based on a single protocol (extraction, trypsin digestion, and purification) applicable to the different tested foodstuffs: chocolate, ice cream, tomato sauce, and processed cookies. The determined limits of quantitation, expressed in total milk, egg, peanut, or soy proteins (and not soluble proteins) per kilogram of food, are: 0.5mg/kg for milk (detection of caseins), 5mg/kg for milk (detection of whey), 2.5mg/kg for peanut, 5mg/kg for soy, 3.4mg/kg for egg (detection of egg white), and 30.8mg/kg for egg (detection of egg yolk). The main advantage is the ability of the method to detect four major food allergens simultaneously in processed and complex matrices with very high sensitivity and specificity.
langue originaleAnglais
Pages (de - à)115-123
Nombre de pages9
journalJournal of Chromatography A
Volume1464
Date de mise en ligne précoce18 août 2016
Les DOIs
Etat de la publicationPublié - 18 août 2016

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  • RAFA 2017

    Mélanie Planque (Poster) & Thierry Arnould (Poster)

    7 nov. 201710 nov. 2017

    Activité: Types de Participation ou d'organisation d'un événementParticipation à un Colloque, une journée d'étude

  • Trends in Food Analysis VIII

    Mélanie Planque (Conférencier) & Thierry Arnould (Poster)

    24 mai 2017

    Activité: Types de Participation ou d'organisation d'un événementParticipation à une conférence, un congrès

  • Trends in Food Analysis VIII

    Mélanie Planque (Poster), Thierry Arnould (Poster), Marc Dieu (Poster), Philippe Delahaut (Poster), Patricia Renard (Poster) & Nathalie Gillard (Poster)

    24 mai 2017

    Activité: Types de Participation ou d'organisation d'un événementParticipation à une conférence, un congrès

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