Adherence and interaction of cationic quantum dots on bacterial surfaces

Cheng Yang, Hao Xie, Qi Chang Li, En Jie Sun, Bao Lian Su

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Understanding molecular mechanisms of interactions between nanoparticles and bacteria is important and essential to develop nanotechnology for medical and environmental applications. Quantum dots (QDs) are specific nanoparticles with unique optical properties and high photochemical stability. In the present study, direct interactions were observed between cationic QDs and bacteria. Distinct fluorescence quenching patterns were developed when cationic QDs interacted with Gram negative and Gram positive bacteria. The aggregation of QDs on bacterial surface as well as fluorescence quenching depends upon the chemical composition and structure of the bacterial cell envelopes. The presence of lipopolysaccharide is unique to Gram-negative bacterial surface and provides negatively charge areas for absorbing cationic QDs. The effects of lipopolysaccharide were proved on fluorescence quenching of cationic QDs. In contrast to Gram-negative bacteria, the presence of teichoic acids is unique to Gram-positive bacterial cell wall and provides negatively charged sites for cationic QDs along the chain of teichoic-acid molecules, which may protect QDs from aggregation and fluorescence quenching. This study may not only provide insight into behaviors of QDs on bacterial cell surfaces but also open a new avenue for designing and applying QDs as biosensors in microbiology, medicine, and environmental science.

langue originaleAnglais
Pages (de - à)388-395
Nombre de pages8
journalJournal of Colloid and Interface Science
Volume450
Les DOIs
étatPublié - 5 juil. 2015

Empreinte digitale

Semiconductor quantum dots
Quenching
Bacteria
Fluorescence
Teichoic Acids
Lipopolysaccharides
Agglomeration
Nanoparticles
Microbiology
Acids
Nanotechnology
Biosensors
Medicine
Optical properties
Cells
Molecules
Chemical analysis

Citer ceci

Yang, Cheng ; Xie, Hao ; Li, Qi Chang ; Sun, En Jie ; Su, Bao Lian. / Adherence and interaction of cationic quantum dots on bacterial surfaces. Dans: Journal of Colloid and Interface Science. 2015 ; Vol 450. p. 388-395.
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abstract = "Understanding molecular mechanisms of interactions between nanoparticles and bacteria is important and essential to develop nanotechnology for medical and environmental applications. Quantum dots (QDs) are specific nanoparticles with unique optical properties and high photochemical stability. In the present study, direct interactions were observed between cationic QDs and bacteria. Distinct fluorescence quenching patterns were developed when cationic QDs interacted with Gram negative and Gram positive bacteria. The aggregation of QDs on bacterial surface as well as fluorescence quenching depends upon the chemical composition and structure of the bacterial cell envelopes. The presence of lipopolysaccharide is unique to Gram-negative bacterial surface and provides negatively charge areas for absorbing cationic QDs. The effects of lipopolysaccharide were proved on fluorescence quenching of cationic QDs. In contrast to Gram-negative bacteria, the presence of teichoic acids is unique to Gram-positive bacterial cell wall and provides negatively charged sites for cationic QDs along the chain of teichoic-acid molecules, which may protect QDs from aggregation and fluorescence quenching. This study may not only provide insight into behaviors of QDs on bacterial cell surfaces but also open a new avenue for designing and applying QDs as biosensors in microbiology, medicine, and environmental science.",
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Adherence and interaction of cationic quantum dots on bacterial surfaces. / Yang, Cheng; Xie, Hao; Li, Qi Chang; Sun, En Jie; Su, Bao Lian.

Dans: Journal of Colloid and Interface Science, Vol 450, 05.07.2015, p. 388-395.

Résultats de recherche: Contribution à un journal/une revueArticle

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