A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium

T Michiels, M Y Popoff, S Durviaux, C Coynault, G Cornelis

    Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

    Résumé

    A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
    langue originaleAnglais
    Pages (de - à)109-16
    Nombre de pages8
    journalMicrobial pathogenesis
    Volume3
    Numéro de publication2
    Etat de la publicationPublié - 1987

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