TY - JOUR
T1 - A new classification of pre-ovulatory oocyte maturation stages in pikeperch, Sander lucioperca (L.), and its application during artificial reproduction
AU - Zarski, D.
AU - Kucharczyk, D.
AU - Targońska, K.
AU - Kupren, K.
AU - Palińska, K.
AU - Fontaine, P.
AU - Kestemont, P.
N1 - Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/4/1
Y1 - 2012/4/1
N2 - This study aimed at improving the reproduction effectiveness and synchronization of ovulation in the pikeperch, Sander lucioperca (L.), during induced spawning, which is one of the main bottlenecks in the aquaculture of this species. For this purpose, a new categorization of maturation stages in pre-ovulatory oocytes was applied. It is generally based on two morphological indicators: germinal vesicle migration or its breakdown (GVBD) and different oil droplet coalescence rates. This categorization covered seven stages (from I to VII) - from the end of vitellogenesis to ovulation. The categorization was verified by controlled reproduction with the use of hormonal stimulation (500IU of hCG per kg of female body weight) and low spawning temperature (12°C), which extended the latency time. In addition, some morphological indicators (pseudo-gonadosomatic index, Fulton's condition coefficient) of females were calculated in order to determine their usability in determining the maturation stage. However, these indicators proved to be ineffective for this purpose, further highlighting the need to determine the maturational stages in pre-ovulatory oocytes to synchronize ovulation in pikeperch. During the experiment, ovulation seemed to be synchronized among the experimental treatments. Statistical differences were found in terms of latency time between experimental groups at different maturity stages (II - 78-98h; III - 57-78h; IV - 48-58h; V - 32-49h; VI - 5-30h) according to the proposed classification. This classification and the results presented in the study significantly improved the synchronization of ovulation, which may positively affect the effectiveness of pikeperch production under controlled conditions.
AB - This study aimed at improving the reproduction effectiveness and synchronization of ovulation in the pikeperch, Sander lucioperca (L.), during induced spawning, which is one of the main bottlenecks in the aquaculture of this species. For this purpose, a new categorization of maturation stages in pre-ovulatory oocytes was applied. It is generally based on two morphological indicators: germinal vesicle migration or its breakdown (GVBD) and different oil droplet coalescence rates. This categorization covered seven stages (from I to VII) - from the end of vitellogenesis to ovulation. The categorization was verified by controlled reproduction with the use of hormonal stimulation (500IU of hCG per kg of female body weight) and low spawning temperature (12°C), which extended the latency time. In addition, some morphological indicators (pseudo-gonadosomatic index, Fulton's condition coefficient) of females were calculated in order to determine their usability in determining the maturation stage. However, these indicators proved to be ineffective for this purpose, further highlighting the need to determine the maturational stages in pre-ovulatory oocytes to synchronize ovulation in pikeperch. During the experiment, ovulation seemed to be synchronized among the experimental treatments. Statistical differences were found in terms of latency time between experimental groups at different maturity stages (II - 78-98h; III - 57-78h; IV - 48-58h; V - 32-49h; VI - 5-30h) according to the proposed classification. This classification and the results presented in the study significantly improved the synchronization of ovulation, which may positively affect the effectiveness of pikeperch production under controlled conditions.
UR - http://www.scopus.com/inward/record.url?scp=84859731396&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2109.2011.02879.x
DO - 10.1111/j.1365-2109.2011.02879.x
M3 - Article
AN - SCOPUS:84859731396
SN - 0270-7306
VL - 43
SP - 713
EP - 721
JO - Molecular Cell Biology
JF - Molecular Cell Biology
IS - 5
ER -