A multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol film modified carbon paste electrode for the amperometric determination of l-tyrosine

Ozma J. D'Souza, Ronald J. Mascarenhas, Ashis K. Satpati, Irishi N N Namboothiri, Simon Detriche, Zineb Mekhalif, Joseph Delhalle

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

A novel multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol (DCPIP) film modified carbon paste electrode (MCPE/MWCNTs/p-DCPIP) was constructed using drop casting and electropolymerization methods. Cyclic voltammetry and amperometric techniques were employed for the determination of l-tyrosine (Tyr). An electrochemically induced oxa-Michael addition reaction has been proposed as a possible mechanism of the electropolymerization of DCPIP on the surface of the modified electrode. The surface morphology and interface properties of the electrodes were investigated by FE-SEM images and the electrochemical impedance spectroscopy (EIS), respectively. The AFM images confirm the formation of nano fibrous deposits of DCPIP. The spectroelectrochemical study performed reveals the structural changes in Tyr during oxidation. This sensor was used for the selective detection of Tyr at physiological pH in the presence of some common interfering biomolecules. Chronoamperometic technique was employed for the determination of the diffusion coefficient and the standard heterogeneous electron transfer rate constant (k0) of Tyr at the modified electrode. The sensor exhibited a linear response to Tyr over a wide concentration range of 0.3 μM-8.0 μM and 10.0 μM-110.0 μM with a detection limit (70.5 ± 2.35) nM (S/N = 3). The practical utility of the sensor was demonstrated by determining Tyr in spiked human blood serum and soya sauce.

langueAnglais
Pages91472-91481
Nombre de pages10
journalRSC Advances
Volume5
Numéro111
Les DOIs
étatPublié - 2015

Empreinte digitale

2,6-Dichloroindophenol
Carbon Nanotubes
Ointments
Tyrosine
Carbon nanotubes
Carbon
Electrodes
Electropolymerization
Sensors
Addition reactions
Biomolecules
Electrochemical impedance spectroscopy
Cyclic voltammetry
Surface morphology
Rate constants
Casting
Blood
Deposits
Oxidation
Scanning electron microscopy

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title = "A multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol film modified carbon paste electrode for the amperometric determination of l-tyrosine",
abstract = "A novel multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol (DCPIP) film modified carbon paste electrode (MCPE/MWCNTs/p-DCPIP) was constructed using drop casting and electropolymerization methods. Cyclic voltammetry and amperometric techniques were employed for the determination of l-tyrosine (Tyr). An electrochemically induced oxa-Michael addition reaction has been proposed as a possible mechanism of the electropolymerization of DCPIP on the surface of the modified electrode. The surface morphology and interface properties of the electrodes were investigated by FE-SEM images and the electrochemical impedance spectroscopy (EIS), respectively. The AFM images confirm the formation of nano fibrous deposits of DCPIP. The spectroelectrochemical study performed reveals the structural changes in Tyr during oxidation. This sensor was used for the selective detection of Tyr at physiological pH in the presence of some common interfering biomolecules. Chronoamperometic technique was employed for the determination of the diffusion coefficient and the standard heterogeneous electron transfer rate constant (k0) of Tyr at the modified electrode. The sensor exhibited a linear response to Tyr over a wide concentration range of 0.3 μM-8.0 μM and 10.0 μM-110.0 μM with a detection limit (70.5 ± 2.35) nM (S/N = 3). The practical utility of the sensor was demonstrated by determining Tyr in spiked human blood serum and soya sauce.",
author = "D'Souza, {Ozma J.} and Mascarenhas, {Ronald J.} and Satpati, {Ashis K.} and Namboothiri, {Irishi N N} and Simon Detriche and Zineb Mekhalif and Joseph Delhalle",
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doi = "10.1039/c5ra18329d",
language = "English",
volume = "5",
pages = "91472--91481",
journal = "RSC Advances",
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A multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol film modified carbon paste electrode for the amperometric determination of l-tyrosine. / D'Souza, Ozma J.; Mascarenhas, Ronald J.; Satpati, Ashis K.; Namboothiri, Irishi N N; Detriche, Simon; Mekhalif, Zineb; Delhalle, Joseph.

Dans: RSC Advances, Vol 5, Numéro 111, 2015, p. 91472-91481.

Résultats de recherche: Contribution à un journal/une revueArticle

TY - JOUR

T1 - A multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol film modified carbon paste electrode for the amperometric determination of l-tyrosine

AU - D'Souza,Ozma J.

AU - Mascarenhas,Ronald J.

AU - Satpati,Ashis K.

AU - Namboothiri,Irishi N N

AU - Detriche,Simon

AU - Mekhalif,Zineb

AU - Delhalle,Joseph

PY - 2015

Y1 - 2015

N2 - A novel multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol (DCPIP) film modified carbon paste electrode (MCPE/MWCNTs/p-DCPIP) was constructed using drop casting and electropolymerization methods. Cyclic voltammetry and amperometric techniques were employed for the determination of l-tyrosine (Tyr). An electrochemically induced oxa-Michael addition reaction has been proposed as a possible mechanism of the electropolymerization of DCPIP on the surface of the modified electrode. The surface morphology and interface properties of the electrodes were investigated by FE-SEM images and the electrochemical impedance spectroscopy (EIS), respectively. The AFM images confirm the formation of nano fibrous deposits of DCPIP. The spectroelectrochemical study performed reveals the structural changes in Tyr during oxidation. This sensor was used for the selective detection of Tyr at physiological pH in the presence of some common interfering biomolecules. Chronoamperometic technique was employed for the determination of the diffusion coefficient and the standard heterogeneous electron transfer rate constant (k0) of Tyr at the modified electrode. The sensor exhibited a linear response to Tyr over a wide concentration range of 0.3 μM-8.0 μM and 10.0 μM-110.0 μM with a detection limit (70.5 ± 2.35) nM (S/N = 3). The practical utility of the sensor was demonstrated by determining Tyr in spiked human blood serum and soya sauce.

AB - A novel multi-walled carbon nanotube/poly-2,6-dichlorophenolindophenol (DCPIP) film modified carbon paste electrode (MCPE/MWCNTs/p-DCPIP) was constructed using drop casting and electropolymerization methods. Cyclic voltammetry and amperometric techniques were employed for the determination of l-tyrosine (Tyr). An electrochemically induced oxa-Michael addition reaction has been proposed as a possible mechanism of the electropolymerization of DCPIP on the surface of the modified electrode. The surface morphology and interface properties of the electrodes were investigated by FE-SEM images and the electrochemical impedance spectroscopy (EIS), respectively. The AFM images confirm the formation of nano fibrous deposits of DCPIP. The spectroelectrochemical study performed reveals the structural changes in Tyr during oxidation. This sensor was used for the selective detection of Tyr at physiological pH in the presence of some common interfering biomolecules. Chronoamperometic technique was employed for the determination of the diffusion coefficient and the standard heterogeneous electron transfer rate constant (k0) of Tyr at the modified electrode. The sensor exhibited a linear response to Tyr over a wide concentration range of 0.3 μM-8.0 μM and 10.0 μM-110.0 μM with a detection limit (70.5 ± 2.35) nM (S/N = 3). The practical utility of the sensor was demonstrated by determining Tyr in spiked human blood serum and soya sauce.

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JO - RSC Advances

T2 - RSC Advances

JF - RSC Advances

SN - 2046-2069

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