3D correlative electron microscopy reveals continuity of Brucellacontaining vacuoles with the endoplasmic reticulum

Jaroslaw Sedzicki, Therese Tschon, Shyan Huey Low, Kevin Willemart, Kenneth N Goldie, Jean-Jacques Letesson, Henning Stahlberg, Christoph Dehio

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Entry of the facultative intracellular pathogenBrucellainto host cells results in the formation of endosomalBrucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicativeBrucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers; however, the relationship of the vacuole to the genuine ER has remained elusive. Here, we have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion beam/scanning electron microscopic tomography (FIB/SEM). StudyingB. abortus-infected HeLa cells and trophoblasts derived fromB. melitensis-infected mice, we demonstrate that rBCVs are complex and interconnected compartments that are continuous with neighboring ER cisternae, thus supporting a model that rBCVs are extensions of genuine ER.

langue originaleAnglais
Numéro d'articlejcs210799
journalJournal of Cell Science
Volume131
Numéro de publication4
Les DOIs
Etat de la publicationPublié - 1 févr. 2018
Modification externeOui

Empreinte digitale Examiner les sujets de recherche de « 3D correlative electron microscopy reveals continuity of Brucellacontaining vacuoles with the endoplasmic reticulum ». Ensemble, ils forment une empreinte digitale unique.

  • Équipement

  • Thèses de l'étudiant

    Etude du facteur de transcription CHOP10/GADD153 Dans les macrophages RAW264.7 exposés à Brucella abortus

    Author: LOBET, E., 2012

    Superviseur: Arnould, T. (Promoteur)

    Thèse de l'étudiant: Master typesMaster en biochimie et biologie moléculaire et cellulaire

    Fichier

    Contient cette citation