Spectrométrie de masse (MASUN)

DESCRIPTION

The University of Namur Mass Spectrometry platform (MaSUN) involves equipment from different research units ofthe departments of Pharmacy, Chemistry and Biology. Our aim is to provide support in mass spectrometry, proteomicsanalyses, gel electrophoresis to the UNamur students and staff. Our facility is also open to collaborate with otheruniversities and public or private laboratories.

EXPERTISE

Identification and characterization of small molecules.

Targeted compounds quantification (biomarkers, drugs...).

Proteomics analyses (gel-free or gel-dependent, label-free quantification).

Peptidomics (profile analyses).

Two dimensional HPLC (2D-LC).

Gel electrophoresis including 2D-DIGE.

EQUIPMENT

MASS SPECTROMETERS

• LC-MS

• UPLC-MSMS Xevo TQ-S (Waters)

• High resolution nanoRSLC-MSMSmaXis Impact (Bruker)

• Characterization of small molecules

• Biomarker and drug quantification

• Protein identification in complexsamples

GEL-DEPENDENT PROTEOMICS

• ELECTROPHORESIS

• TYPHOON CONFOCAL SCANNERand SPOT PICKER ROBOT

• DECYDER software

• 2D Ettan Dalt electrophoresisdevice

• For fluorescence-labelled 2D gels

• 2D-DIGE image analysis

GEL-FREE PROTEOMICS

• High resolution nanoRSLC-MSMS

• 2D-LC

• Mascot, Scaffold, Progenesis QIsoftwares

• Protein identification in complexmixtures

• Two dimensional HPLC separation

• Proteomics data analysis andquantification