Protection of Wistar-Furth rats from post-ischemic acute renal injury: a role for Nitric Oxide and Thromboxane?

  • Virginie Voisin

Student thesis: Doc typesDoctor of Biomedical and Pharmaceutical Sciences


Wistar-Furth (WF) rats are a strain of rats usually employed in models of full major histocompatibility complex-mismatched kidney transplantation. These were demonstrated to be resistant to several models of chronic kidney disease. In our study, we investigated their potential resistance to a renal ischemia-reperfusion (I/R) injury in comparison to another strain, Wistar-Hanover (WH) rats. Anesthetized male WH and WF rats were submitted to I/R by occlusion of the left renal artery, followed by contralateral nephrectomy. Urine, blood and tissue samples were collected at different time-points post-I/R to evaluate renal function, inflammation and tubular injury along with the determination of the NO synthase expression. In post-ischemic WF rats, the renal function was better preserved than in the post-ischemic WH rats. In addition, the post-ischemic WF rats presented less inflammation than the WH rats. These observations were associated with the maintenance of the nNOS expression along with a lower induction level of iNOS expression in WF rats than in WH rats. Moreover, WF rats excreted also a significant smaller amount of TxB2. In conclusion, our results showed that WF rats were more resistant to an I/R injury than WH rats. This could be explain to the differential regulation of intrarenal NOSs expression as well as the TxA2 production.
Date of Award1 Jul 2014
Original languageFrench
Awarding Institution
  • University of Namur
SupervisorNathalie Caron (Supervisor), Yves Poumay (President), Carine Michiels (Jury), Nathalie Kirschvink (Jury), Bruno Flamion (Jury), Jean-Michel Dogne (Jury), Nadine Bouby (Jury) & Joëlle Nortier (Jury)


  • acute renal injury
  • renal ischemia-reperfusion
  • NO synthases
  • Thromboxane
  • Wistar-Furth rats

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