AbstractThis work is divided in two main parts that present two relatively independent research output produced during my thesis.
In the first part, I explored the causes and consequences of a surprising connection between Pol II phosphorylation and tRNA expression in fission yeast. Related to this part, the Appendix 1 presents a published work assessing the conservation of the functions of the RSC chromatin remodeler (a candidate effector in the Pol II phosphorylation/tRNA expression relationship) in fission yeast and budding yeast (Yague-Sanz et al., 2017).
The second part of the thesis consists in a collaborative project in which we determined the role of the kinase CDK-12 in C. elegans development. With the exception of the first two figures that are required for the general understanding of the study, the presented research represents mostly my contribution to the project, that was limited to the generation and analysis of high throughput sequencing data. Related to this part, the Appendix 2 presents a published bioinformatics pipeline that was specifically developed for the project in order to quantify special mRNA processing events (called spliced leader trans-splicing events) that we found were dependent on the activity of the CDK-12 kinase (Yague-Sanz and Hermand, 2018).
Preceding those two main parts of my thesis, a general introduction summarizes concepts important for the understanding and contextualization of both parts, while smaller introductions within the parts introduce more specific concepts.
|Date of Award||18 Dec 2018|
|Sponsors||Fund for Research Training in Industry and Agriculture (FRIA)|
|Supervisor||Damien Hermand (Supervisor), Marielle Boonen (President), JEAN-PIERRE GILLET (Jury), Helmlinger Dominique (Jury) & Michel Werner (Jury)|
- CTD phosphorylation