Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu

Özgul İnceoğlu, Marc Llirós, Sean A. Crowe, Tamara García-Armisen, Cedric Morana, François Darchambeau, Alberto V. Borges, Jean Pierre Descy, Pierre Servais

Research output: Contribution to journalArticle

Abstract

The microbial community composition in meromictic Lake Kivu, with one of the largest CH<inf>4</inf> reservoirs, was studied using 16S rDNA and ribosomal RNA (rRNA) pyrosequencing during the dry and rainy seasons. Highly abundant taxa were shared in a high percentage between bulk (DNA-based) and active (RNA-based) bacterial communities, whereas a high proportion of rare species was detected only in either an active or bulk community, indicating the existence of a potentially active rare biosphere and the possible underestimation of diversity detected when using only one nucleic acid pool. Most taxa identified as generalists were abundant, and those identified as specialists were more likely to be rare in the bulk community. The overall number of environmental parameters that could explain the variation was higher for abundant taxa in comparison to rare taxa. Clustering analysis based on operational taxonomic units (OTUs at 0.03 cutoff) level revealed significant and systematic microbial community composition shifts with depth. In the oxic zone, Actinobacteria were found highly dominant in the bulk community but not in the metabolically active community. In the oxic–anoxic transition zone, highly abundant potentially active Nitrospira and Methylococcales were observed. The co-occurrence of potentially active sulfur-oxidizing and sulfate-reducing bacteria in the anoxic zone may suggest the presence of an active yet cryptic sulfur cycle.

Original languageEnglish
JournalAquatic Microbial Ecology
DOIs
Publication statusPublished - 26 Apr 2015

Fingerprint

meromictic lake
Methylococcales
community composition
microbial communities
microbial community
vertical distribution
sulfur
Otus
sulfur cycle
lakes
sulfate-reducing bacteria
Actinobacteria
sulfate-reducing bacterium
nucleic acid
rare species
bacterial communities
generalist
transition zone
biosphere
nucleic acids

Keywords

  • Abundant and rare
  • Active
  • Archaea
  • Bacteria
  • Bulk
  • Meromictic lake
  • Network
  • Pyrosequencing
  • qPCR

Cite this

İnceoğlu, Özgul ; Llirós, Marc ; Crowe, Sean A. ; García-Armisen, Tamara ; Morana, Cedric ; Darchambeau, François ; Borges, Alberto V. ; Descy, Jean Pierre ; Servais, Pierre. / Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu. In: Aquatic Microbial Ecology. 2015.
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Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu. / İnceoğlu, Özgul; Llirós, Marc; Crowe, Sean A.; García-Armisen, Tamara; Morana, Cedric; Darchambeau, François; Borges, Alberto V.; Descy, Jean Pierre; Servais, Pierre.

In: Aquatic Microbial Ecology, 26.04.2015.

Research output: Contribution to journalArticle

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AU - İnceoğlu, Özgul

AU - Llirós, Marc

AU - Crowe, Sean A.

AU - García-Armisen, Tamara

AU - Morana, Cedric

AU - Darchambeau, François

AU - Borges, Alberto V.

AU - Descy, Jean Pierre

AU - Servais, Pierre

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