Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

Quentin Ledoux; Pascal Veys; Pierre Van Cutsem; Sergio Mauro; Fabio Lucaccioni; Istvan Marko

doi:10.4161/psb.26827

Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

Quentin Ledoux, Pascal Veys, Pierre Van Cutsem, Sergio Mauro, Fabio Lucaccioni, Istvan Marko

Unit of Research in Plant Cellular and Molecular Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H₂O₂-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H₂O₂, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H₂O₂ production by plants using spectrofluorometry and microscopy.

Original language	English
Article number	e26827
Journal	Plant Signaling and Behavior
Volume	8
Issue number	11
DOIs	https://doi.org/10.4161/psb.26827
Publication status	Published - 3 Dec 2013

Keywords

Arabidopsis
Cells suspension
ContPY1
Fluorescence
Hydrogen peroxide
Microscopy
Protoplasts

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10.4161/psb.26827

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title = "Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants",

abstract = "Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.",

keywords = "Arabidopsis, Cells suspension, ContPY1, Fluorescence, Hydrogen peroxide, Microscopy, Protoplasts",

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AU - Ledoux, Quentin

AU - Veys, Pascal

AU - Van Cutsem, Pierre

AU - Mauro, Sergio

AU - Lucaccioni, Fabio

AU - Marko, Istvan

PY - 2013/12/3

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N2 - Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.

AB - Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.

KW - Arabidopsis

KW - Cells suspension

KW - ContPY1

KW - Fluorescence

KW - Hydrogen peroxide

KW - Microscopy

KW - Protoplasts

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JF - Plant Signaling and Behavior

SN - 1559-2316

IS - 11

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ER -

Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

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Keywords

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