Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants

Quentin Ledoux, Pascal Veys, Pierre Van Cutsem, Sergio Mauro, Fabio Lucaccioni, Istvan Marko

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Abstract

Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy.

Original languageEnglish
Article numbere26827
JournalPlant Signaling and Behavior
Volume8
Issue number11
DOIs
Publication statusPublished - 3 Dec 2013

Keywords

  • Arabidopsis
  • Cells suspension
  • ContPY1
  • Fluorescence
  • Hydrogen peroxide
  • Microscopy
  • Protoplasts

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