TY - JOUR
T1 - Uptake, tissue distribution and toxicological effects of environmental microplastics in early juvenile fish Dicentrarchus labrax
AU - Zitouni, Nesrine
AU - Bousserrhine, Noureddine
AU - Missawi, Omayma
AU - Boughattas, Iteb
AU - Chèvre, Nathalie
AU - Santos, Raphael
AU - Belbekhouche, Sabrina
AU - Alphonse, Vanessa
AU - Tisserand, Floriane
AU - Balmassiere, Ludivine
AU - Dos Santos, Sofia Pereira
AU - Mokni, Moncef
AU - Guerbej, Hamadi
AU - Banni, Mohamed
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2/5
Y1 - 2021/2/5
N2 - As the smallest environmental microplastics (EMPs), even at nanoscale, are increasingly present in the environment, their availability and physical and chemical effects on marine organisms are poorly documented. In the present study, we primarily investigated the uptake and accumulation of a mixture of environmental microplastics (EMPs) obtained during an artificial degradation process in early-juvenile sea bass (Dicentrarchus labrax). Moreover, we evaluated their hazardous effects using biochemical markers of cytotoxicity. Polymer distribution and composition in gill, gut, and liver were analyzed using polarized light microscopy (PLM) and Raman microspectroscopy (RMS). Our findings revealed the size-dependent ingestion and accumulation of smaller MPs (0.45–3 µm) in fish tissues even after a short-term exposure (3 and 5 days). In addition to MPs, our results showed the presence of plastic additives including plasticizers, flame retardants, curing agents, heat stabilizers, and fiber-reinforced plastic materials in fish tissues, which contributed mostly to the larger-sized range (≥ 1.2 µm). Our data showed that significant oxidative alterations were highly correlated with MPs size range. Our results emphasized that the toxicity of smaller EMPs (≤ 3 µm) was closely related to different factors, including the target tissue, exposure duration, size range of MPs, and their chemical properties.
AB - As the smallest environmental microplastics (EMPs), even at nanoscale, are increasingly present in the environment, their availability and physical and chemical effects on marine organisms are poorly documented. In the present study, we primarily investigated the uptake and accumulation of a mixture of environmental microplastics (EMPs) obtained during an artificial degradation process in early-juvenile sea bass (Dicentrarchus labrax). Moreover, we evaluated their hazardous effects using biochemical markers of cytotoxicity. Polymer distribution and composition in gill, gut, and liver were analyzed using polarized light microscopy (PLM) and Raman microspectroscopy (RMS). Our findings revealed the size-dependent ingestion and accumulation of smaller MPs (0.45–3 µm) in fish tissues even after a short-term exposure (3 and 5 days). In addition to MPs, our results showed the presence of plastic additives including plasticizers, flame retardants, curing agents, heat stabilizers, and fiber-reinforced plastic materials in fish tissues, which contributed mostly to the larger-sized range (≥ 1.2 µm). Our data showed that significant oxidative alterations were highly correlated with MPs size range. Our results emphasized that the toxicity of smaller EMPs (≤ 3 µm) was closely related to different factors, including the target tissue, exposure duration, size range of MPs, and their chemical properties.
KW - Early-juvenile sea bass
KW - Environmental microplastics
KW - Tissue distribution
KW - Toxicity
KW - Uptake
UR - http://www.scopus.com/inward/record.url?scp=85091970317&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2020.124055
DO - 10.1016/j.jhazmat.2020.124055
M3 - Article
C2 - 33265060
AN - SCOPUS:85091970317
SN - 0304-3894
VL - 403
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 124055
ER -