Human diploid fibroblasts undergo premature senescence after treatment with sublethal concentration of H2O2. We report the first proteomic study of microsomal proteins in the context of H2O2-induced premature senescence by using 2D-DIGE approach. Twelve different proteins with altered abundance at day 3 after treatment with H2O2 were identified. Among them, we demonstrated a re-localization of annexin A2 in plasma membrane. © 2008 Elsevier Inc. All rights reserved.