Projects per year
Here, we demonstrate how sum frequency generation (SFG), a vibrational spectroscopy based on a nonlinear three-photon mixing process, may provide a direct and unique fingerprint of bio-recognition; This latter can be detected with an intrinsically discriminating unspecific adsorption, thanks to the high sensitivity of the second-order nonlinear optical (NLO) response to preferential molecular orientation and symmetry properties. As a proof of concept, we have detected the biological event at the solid/liquid interface of a model bio-active antigen platform, based on a solid-supported hybrid lipid bilayer (ss-HLB) of a 2,4-dinitrophenyl (DNP) lipid, towards a monoclonal mouse anti-DNP complementary antibody.
- antigen–antibody recognition
- lipid bilayers
- sum frequency generation
- vibrational spectroscopy
Action Nationale de Formation du CNRS en "Spectroscopie et Imagerie d’interfaces materiaux- molecules du vivant"
Francesca Cecchet (Invited Speaker)15 Oct 2014 → 17 Oct 2014
Activity: Participating in or organising an event types › Participation in conference