Ultrastructural features of hepatocytes in cultured Eurasian perch (Perca fluviatilis L.) as affected by nutritional and husbandry conditions, doi:10.1017/S0007114508926532

Gersande Blanchard, Jean-N. GARDEUR, Nicolas MATHIS, Jean BRUN-BELLUT, Patrick Kestemont

Research output: Contribution to journalArticle

Abstract

A wide range of factors can be attributed to the syndrome of fatty liver observed in some cultured fish species. The objective of the study was therefore to quantify different hepatocyte ultrastructural features as potentially influenced by twelve nutritional and husbandry factors, in order to discriminate the most influent factors in Eurasian perch (Perca fluviatilis), a typical carnivorous temperate fish species. Twenty-four groups of juveniles (initial weight 57·6 (SD 14·4) g) were intensively reared for 116 d and fed sixteen different isoproteic diets. The distribution of the experimental treatments was based on a multivariate fractional factorial design (L24 212) with either high (þ1) or low (21) level of each of the following factors: diet (lipid and protein sources, lipid content, astaxanthin enrichment), feeding level, daily and weekly distribution frequency, fish density, initial weight heterogeneity, temperature, photoperiod, and light spectrum. Liver lipid droplets, glycogen, mitochondria and rough endoplasmic reticulum (RER) were semi-quantified and analysed by a soft imaging system using transmission electronic microscopy photographs. Important variability of hepatocyte ultrastructural features was observed. The present study confirms that the rearing temperature, through its influences in the general metabolic activity, seems to be the main factor modifying mainly lipid droplet accumulation and RER development. However, factors that could be pooled under the designation of factors leading to food accessibility and lipid and protein quality intensify or compensate the effect of temperature.
Original languageEnglish
Pages (from-to)317-331
Number of pages15
JournalBritish Journal of Nutrition
Volume100
Issue number2
Publication statusPublished - 2008

Fingerprint

Perches
Hepatocytes
Fishes
Rough Endoplasmic Reticulum
Lipids
Temperature
Diet
Weights and Measures
Photoperiod
Fatty Liver
Glycogen
Microscopy
Mitochondria
Proteins
Light
Food
Liver
Lipid Droplets
Therapeutics

Keywords

  • Perca fluviatilis: Ultrastructural features of hepatocytes: Nutritional and husbandry factors: Fractional factorial design

Cite this

@article{d80ac2f1bd894bfd886b3047f0eb5231,
title = "Ultrastructural features of hepatocytes in cultured Eurasian perch (Perca fluviatilis L.) as affected by nutritional and husbandry conditions, doi:10.1017/S0007114508926532",
abstract = "A wide range of factors can be attributed to the syndrome of fatty liver observed in some cultured fish species. The objective of the study was therefore to quantify different hepatocyte ultrastructural features as potentially influenced by twelve nutritional and husbandry factors, in order to discriminate the most influent factors in Eurasian perch (Perca fluviatilis), a typical carnivorous temperate fish species. Twenty-four groups of juveniles (initial weight 57·6 (SD 14·4) g) were intensively reared for 116 d and fed sixteen different isoproteic diets. The distribution of the experimental treatments was based on a multivariate fractional factorial design (L24 212) with either high ({\th}1) or low (21) level of each of the following factors: diet (lipid and protein sources, lipid content, astaxanthin enrichment), feeding level, daily and weekly distribution frequency, fish density, initial weight heterogeneity, temperature, photoperiod, and light spectrum. Liver lipid droplets, glycogen, mitochondria and rough endoplasmic reticulum (RER) were semi-quantified and analysed by a soft imaging system using transmission electronic microscopy photographs. Important variability of hepatocyte ultrastructural features was observed. The present study confirms that the rearing temperature, through its influences in the general metabolic activity, seems to be the main factor modifying mainly lipid droplet accumulation and RER development. However, factors that could be pooled under the designation of factors leading to food accessibility and lipid and protein quality intensify or compensate the effect of temperature.",
keywords = "Perca fluviatilis: Ultrastructural features of hepatocytes: Nutritional and husbandry factors: Fractional factorial design",
author = "Gersande Blanchard and Jean-N. GARDEUR and Nicolas MATHIS and Jean BRUN-BELLUT and Patrick Kestemont",
year = "2008",
language = "English",
volume = "100",
pages = "317--331",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "2",

}

TY - JOUR

T1 - Ultrastructural features of hepatocytes in cultured Eurasian perch (Perca fluviatilis L.) as affected by nutritional and husbandry conditions, doi:10.1017/S0007114508926532

AU - Blanchard, Gersande

AU - GARDEUR, Jean-N.

AU - MATHIS, Nicolas

AU - BRUN-BELLUT, Jean

AU - Kestemont, Patrick

PY - 2008

Y1 - 2008

N2 - A wide range of factors can be attributed to the syndrome of fatty liver observed in some cultured fish species. The objective of the study was therefore to quantify different hepatocyte ultrastructural features as potentially influenced by twelve nutritional and husbandry factors, in order to discriminate the most influent factors in Eurasian perch (Perca fluviatilis), a typical carnivorous temperate fish species. Twenty-four groups of juveniles (initial weight 57·6 (SD 14·4) g) were intensively reared for 116 d and fed sixteen different isoproteic diets. The distribution of the experimental treatments was based on a multivariate fractional factorial design (L24 212) with either high (þ1) or low (21) level of each of the following factors: diet (lipid and protein sources, lipid content, astaxanthin enrichment), feeding level, daily and weekly distribution frequency, fish density, initial weight heterogeneity, temperature, photoperiod, and light spectrum. Liver lipid droplets, glycogen, mitochondria and rough endoplasmic reticulum (RER) were semi-quantified and analysed by a soft imaging system using transmission electronic microscopy photographs. Important variability of hepatocyte ultrastructural features was observed. The present study confirms that the rearing temperature, through its influences in the general metabolic activity, seems to be the main factor modifying mainly lipid droplet accumulation and RER development. However, factors that could be pooled under the designation of factors leading to food accessibility and lipid and protein quality intensify or compensate the effect of temperature.

AB - A wide range of factors can be attributed to the syndrome of fatty liver observed in some cultured fish species. The objective of the study was therefore to quantify different hepatocyte ultrastructural features as potentially influenced by twelve nutritional and husbandry factors, in order to discriminate the most influent factors in Eurasian perch (Perca fluviatilis), a typical carnivorous temperate fish species. Twenty-four groups of juveniles (initial weight 57·6 (SD 14·4) g) were intensively reared for 116 d and fed sixteen different isoproteic diets. The distribution of the experimental treatments was based on a multivariate fractional factorial design (L24 212) with either high (þ1) or low (21) level of each of the following factors: diet (lipid and protein sources, lipid content, astaxanthin enrichment), feeding level, daily and weekly distribution frequency, fish density, initial weight heterogeneity, temperature, photoperiod, and light spectrum. Liver lipid droplets, glycogen, mitochondria and rough endoplasmic reticulum (RER) were semi-quantified and analysed by a soft imaging system using transmission electronic microscopy photographs. Important variability of hepatocyte ultrastructural features was observed. The present study confirms that the rearing temperature, through its influences in the general metabolic activity, seems to be the main factor modifying mainly lipid droplet accumulation and RER development. However, factors that could be pooled under the designation of factors leading to food accessibility and lipid and protein quality intensify or compensate the effect of temperature.

KW - Perca fluviatilis: Ultrastructural features of hepatocytes: Nutritional and husbandry factors: Fractional factorial design

M3 - Article

VL - 100

SP - 317

EP - 331

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 2

ER -