Transposon sequencing of Brucella abortus uncovers essential genes for growth in vitro and inside macrophages

Research output: Contribution to journalArticle

Abstract

Brucella abortus is a class III zoonotic bacterial pathogen able to survive and replicate inside host cells, including macrophages. Here we report a multidimensional transposon sequencing analysis to identify genes essential for Brucella abortus growth in rich medium and replication in RAW 264.7 macrophages. The construction of a dense transposon mutant library and mapping of 929,769 unique mini-Tn5 insertion sites in the genome allowed identification of 491 essential coding sequences and essential segments in the B. abortus genome. Chromosome II carries a lower proportion (5%) of essential genes than chromosome I (19%), supporting the hypothesis of a recent acquisition of a megaplasmid as the origin of chromosome II. Temporally resolved transposon sequencing analysis as a function of macrophage infection stages identified 79 genes with a specific attenuation phenotype in macrophages, at either 2, 5, or 24 h postinfection, and 86 genes for which the attenuated mutant phenotype correlated with a growth defect on plates. We identified 48 genes required for intracellular growth, including the virB operon, encoding the type IV secretion system, which supports the validity of the screen. The remaining genes encode amino acid and pyrimidine biosynthesis, electron transfer systems, transcriptional regulators, and transporters. In particular, we report the need of an intact pyrimidine nucleotide biosynthesis pathway in order for B. abortus to proliferate inside RAW 264.7 macrophages.

Original languageEnglish
Article numbere00312-18
JournalInfection and Immunity
Volume86
Issue number8
Early online date2018
DOIs
Publication statusPublished - 1 Aug 2018
Externally publishedYes

Fingerprint

Brucella abortus
Essential Genes
Macrophages
Growth
Genes
Chromosomes
Pyrimidine Nucleotides
Genome
Phenotype
Chromosomes, Human, Pair 19
Zoonoses
Operon
In Vitro Techniques
Electrons
Amino Acids
Infection

Keywords

  • Brucella
  • RAW264.7 macrophage
  • Tn-seq

Cite this

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title = "Transposon sequencing of Brucella abortus uncovers essential genes for growth in vitro and inside macrophages",
abstract = "Brucella abortus is a class III zoonotic bacterial pathogen able to survive and replicate inside host cells, including macrophages. Here we report a multidimensional transposon sequencing analysis to identify genes essential for Brucella abortus growth in rich medium and replication in RAW 264.7 macrophages. The construction of a dense transposon mutant library and mapping of 929,769 unique mini-Tn5 insertion sites in the genome allowed identification of 491 essential coding sequences and essential segments in the B. abortus genome. Chromosome II carries a lower proportion (5{\%}) of essential genes than chromosome I (19{\%}), supporting the hypothesis of a recent acquisition of a megaplasmid as the origin of chromosome II. Temporally resolved transposon sequencing analysis as a function of macrophage infection stages identified 79 genes with a specific attenuation phenotype in macrophages, at either 2, 5, or 24 h postinfection, and 86 genes for which the attenuated mutant phenotype correlated with a growth defect on plates. We identified 48 genes required for intracellular growth, including the virB operon, encoding the type IV secretion system, which supports the validity of the screen. The remaining genes encode amino acid and pyrimidine biosynthesis, electron transfer systems, transcriptional regulators, and transporters. In particular, we report the need of an intact pyrimidine nucleotide biosynthesis pathway in order for B. abortus to proliferate inside RAW 264.7 macrophages.",
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author = "Jean-Fran{\cc}ois Sternon and Pierre Godessart and {Gon{\cc}alves de Freitas}, Rosa and {Van der Henst}, Mathilde and Katy Poncin and Nayla Francis and Kevin Willemart and Matthias Christen and Beat Christen and Jean-Jacques Letesson and {De Bolle}, Xavier",
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AU - Sternon, Jean-François

AU - Godessart, Pierre

AU - Gonçalves de Freitas, Rosa

AU - Van der Henst, Mathilde

AU - Poncin, Katy

AU - Francis, Nayla

AU - Willemart, Kevin

AU - Christen, Matthias

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AU - De Bolle, Xavier

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