Toward computer simulation of high-LET in vitro survival curves

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Abstract

We developed a Monte Carlo based computer program called MCSC (Monte Carlo Survival Curve) able to predict the survival fraction of cells irradiated in vitro with a broad beam of high linear energy transfer particles. Three types of cell responses are studied: the usual high dose response, the bystander effect and the low-dose hypersensitivity (HRS). The program models the broad beam irradiation and double strand break distribution following Poisson statistics. The progression of cells through the cell cycle is taken into account while the repair takes place. Input parameters are experimentally determined for A549 lung carcinoma cells irradiated with 10 and 20 keV µm(-1) protons, 115 keV µm(-1) alpha particles and for EAhy926 endothelial cells exposed to 115 keV µm(-1) alpha particles. Results of simulations are presented and compared with experimental survival curves obtained for A549 and EAhy296 cells. Results are in good agreement with experimental data for both cell lines and all irradiation protocols. The benefits of MCSC are several: the gain of time that would have been spent performing time-consuming clonogenic assays, the capacity to estimate survival fraction of cell lines not forming colonies and possibly the evaluation of radiosensitivity parameters of given individuals.
Original languageEnglish
Pages (from-to)6495-6510
Number of pages16
JournalPhysics in Medicine and Biology
Volume58
Issue number18
DOIs
Publication statusPublished - 2013

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  • Equipment

    Morphology - Imaging

    Charles Nicaise (Manager) & Francesca Cecchet (Manager)

    Technological Platform Morphology - Imaging

    Facility/equipment: Technological Platform

  • Activities

    • 2 Participation in conference

    International Conference "Radiation Damage in Biomolecular Systems: Nanoscale Insights into Ion-Beam Cancer Therapy" (Nano-IBCT 2014)

    Stéphane Lucas (Speaker)

    27 Oct 201431 Oct 2014

    Activity: Participating in or organising an event typesParticipation in conference

    2nd BHTC workshop ; Hadrontherapy: from cell to patient

    Stéphane Lucas (Organiser)

    23 May 2014

    Activity: Participating in or organising an event typesParticipation in conference

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