Tools for photomotor response assay standardization in ecotoxicological studies: Example of exposure to gentamicin in the freshwater planaria Schmidtea mediterranea

Anthony G.E. Mathiron, Lucia Rejo, Florian Chapeau, Jean Michel Malgouyres, Frédéric Silvestre, Caroline Vignet

Research output: Contribution to journalArticlepeer-review

Abstract

Photomotor response assay (PMR) is very useful in an ecotoxicological context because it allows evaluation of behavioral response to potential toxic compounds. However, a lack of procedure standardization makes results comparison difficult between labs and organisms. Here, we aimed to propose five different tools to standardize the PMR procedure so that it may be applied to all model species, regarding: (1) the minimum total sample size, (2) the acclimation period, (3) the number and duration of light and dark phases alternation, (4) the measured behavior, and (5) the statistical analysis. As an example of procedure application, we analyzed the effect of an exposure to the antibiotic gentamicin on the locomotion behavior during PMR in an invertebrate species: the asexual freshwater planaria Schmidtea mediterranea. We encourage future studies using PMR to follow these five tools to improve data analysis and results comparability.

Original languageEnglish
Article number104242
JournalEnvironmental Toxicology and Pharmacology
Volume102
DOIs
Publication statusPublished - Sept 2023

Keywords

  • Behavior
  • Invertebrate
  • Linear mixed models
  • Locomotion
  • Pharmaceuticals
  • R software

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