TY - JOUR
T1 - Thymus satureioides and Origanum majorana essential oils improve the quality of Beni Arouss buck semen during storage at 4°C
AU - Kchikich, A.
AU - Kirschvink, N.
AU - El Kadili, S.
AU - Raes, M.
AU - El Otmani, S.
AU - Bister, J. L.
AU - El Amiri, B.
AU - Barrijal, S.
AU - Chentouf, M.
N1 - Funding Information:
This research was supported by the Belgian Academy of Research and Higher Education—Research Project for Development (ARES-PRD). The authors thank the staff of National Institute of Agricultural Research, Regional Center of Tangier for their support and collaboration.
Funding Information:
This research was supported by the Belgian Academy of Research and Higher Education—Research Project for Development (ARES‐PRD). The authors thank the staff of National Institute of Agricultural Research, Regional Center of Tangier for their support and collaboration.
Publisher Copyright:
© 2021 Wiley-VCH GmbH.
PY - 2021/12
Y1 - 2021/12
N2 - This study aims to investigate the effects of essential oils (EOs), extracted from Thymus satureioides (TS) and Origanum majorana (OM), on Beni Arouss buck semen quality stored in skimmed milk at 4°C. EOs were extracted by hydro-distillation, and the chemical compounds were determined. Ejaculates were collected from six Beni Arouss bucks, once a week for 10 weeks, and they were pooled, divided into five equal aliquots and diluted to 400 × 106 sperm/ml with skimmed milk supplemented with 0.01% of OM EO, 0.01% of TS EO, 0.05% of OM EO and 0.05% of TS EO. Non-supplemented skimmed milk was considered as a control. Semen motility, kinematic parameters, viability, abnormality, membrane integrity and lipid peroxidation were evaluated at 0, 4, 8, 24, 28, 32 and 48 hr of liquid storage at 4°C. The main EO components were carvacrol (31.7%), thymol (28.0%) and borneol (14.4%) for TS, and terpinene-4-ol (31.2%), γ-terpinene (17.4%) and α-terpinene (12.7%) for OM. The results highlighted a dose-dependent effect of TS and OM EOs on all semen quality parameters. 0.01% of both EOs had a beneficial effect on the sperm preservation stored at 4°C compared with control (p <.05) excepted for the straight-line velocity. The 0.05% EO addition had harmful effects during storage particularly for TS EO. In conclusion, 0.01% of TS and OM EOs are recommended to improve the Beni Arouss buck semen preservation at 4°C.
AB - This study aims to investigate the effects of essential oils (EOs), extracted from Thymus satureioides (TS) and Origanum majorana (OM), on Beni Arouss buck semen quality stored in skimmed milk at 4°C. EOs were extracted by hydro-distillation, and the chemical compounds were determined. Ejaculates were collected from six Beni Arouss bucks, once a week for 10 weeks, and they were pooled, divided into five equal aliquots and diluted to 400 × 106 sperm/ml with skimmed milk supplemented with 0.01% of OM EO, 0.01% of TS EO, 0.05% of OM EO and 0.05% of TS EO. Non-supplemented skimmed milk was considered as a control. Semen motility, kinematic parameters, viability, abnormality, membrane integrity and lipid peroxidation were evaluated at 0, 4, 8, 24, 28, 32 and 48 hr of liquid storage at 4°C. The main EO components were carvacrol (31.7%), thymol (28.0%) and borneol (14.4%) for TS, and terpinene-4-ol (31.2%), γ-terpinene (17.4%) and α-terpinene (12.7%) for OM. The results highlighted a dose-dependent effect of TS and OM EOs on all semen quality parameters. 0.01% of both EOs had a beneficial effect on the sperm preservation stored at 4°C compared with control (p <.05) excepted for the straight-line velocity. The 0.05% EO addition had harmful effects during storage particularly for TS EO. In conclusion, 0.01% of TS and OM EOs are recommended to improve the Beni Arouss buck semen preservation at 4°C.
KW - Buck semen
KW - essential oil
KW - Origanum majorana
KW - preservation
KW - Thymus satureioides
UR - http://www.scopus.com/inward/record.url?scp=85116631831&partnerID=8YFLogxK
U2 - 10.1111/rda.14022
DO - 10.1111/rda.14022
M3 - Article
C2 - 34597454
AN - SCOPUS:85116631831
SN - 0936-6768
VL - 56
SP - 1572
EP - 1581
JO - Reproduction in Domestic Animals
JF - Reproduction in Domestic Animals
IS - 12
ER -