The use of colloidal gold for screening monoclonal antibodies to cell surface antigens

P Coppe; Jean-Jacques Letesson; M Saint-Guillain; R Leloup

The use of colloidal gold for screening monoclonal antibodies to cell surface antigens

P Coppe, Jean-Jacques Letesson, M Saint-Guillain, R Leloup

Research output: Contribution to journal › Article › peer-review

Abstract

An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies is detected by a protein A gold complex. The cell-bound gold can be visualized by either optical or transmission electron microscopy. The results obtained with various monoclonal antibodies are presented.

Original language	English
Pages (from-to)	211-22
Number of pages	12
Journal	Journal of Immunological Methods
Volume	76
Issue number	2
Publication status	Published - 1985

Morphology - Imaging
Francesca Cecchet (Manager) & Henri-Francois Renard (Manager)
Technological Platform Morphology - Imaging
Facility/equipment: Technological Platform

Cite this

@article{01551b5aac68480889e1f8d3636e6460,

title = "The use of colloidal gold for screening monoclonal antibodies to cell surface antigens",

abstract = "An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies is detected by a protein A gold complex. The cell-bound gold can be visualized by either optical or transmission electron microscopy. The results obtained with various monoclonal antibodies are presented.",

author = "P Coppe and Jean-Jacques Letesson and M Saint-Guillain and R Leloup",

year = "1985",

language = "English",

volume = "76",

pages = "211--22",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - The use of colloidal gold for screening monoclonal antibodies to cell surface antigens

AU - Coppe, P

AU - Letesson, Jean-Jacques

AU - Saint-Guillain, M

AU - Leloup, R

PY - 1985

Y1 - 1985

N2 - An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies is detected by a protein A gold complex. The cell-bound gold can be visualized by either optical or transmission electron microscopy. The results obtained with various monoclonal antibodies are presented.

AB - An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies is detected by a protein A gold complex. The cell-bound gold can be visualized by either optical or transmission electron microscopy. The results obtained with various monoclonal antibodies are presented.

M3 - Article

C2 - 3838328

SN - 0022-1759

VL - 76

SP - 211

EP - 222

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

The use of colloidal gold for screening monoclonal antibodies to cell surface antigens

Abstract

Fingerprint

Equipment

Morphology - Imaging

Cite this