The use of colloidal gold for screening monoclonal antibodies to cell surface antigens

P Coppe, Jean-Jacques Letesson, M Saint-Guillain, R Leloup

Research output: Contribution to journalArticlepeer-review

Abstract

An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies is detected by a protein A gold complex. The cell-bound gold can be visualized by either optical or transmission electron microscopy. The results obtained with various monoclonal antibodies are presented.
Original languageEnglish
Pages (from-to)211-22
Number of pages12
JournalJournal of Immunological Methods
Volume76
Issue number2
Publication statusPublished - 1985

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