Abstract
Background: The objective was to evaluate the cytotoxic effect and mechanism of action of vitamins C (VC) and K3 (VK3) on ovarian carcinoma. Materials and Methods: Cytotoxicity assays were performed on ovarian cancer cell lines with VC, VK3 or a VC/VK3 combination. FIC index was employed to evaluate synergism. Flow cytometry was accomplished at 90% cytotoxic doses. Light, transmission electron microscopy and DNA isolation were performed. Results: Antitumor activity was exhibited by both VC, VK3 and VC/VK3 demonstrated synergistic activity. VC/VK3 may induce a G1, block in the cell cycle. Combined vitamin treatment resulted in cells that maintain apparently intact nuclei while extruding pieces of organelle-free cytoplasm. Degradation of chromosomal DNA was observed. Conclusion: Cell death (autoschizis) displayed characteristics of both apoptosis and necrosis. The cytotoxic effects observed may enable vitamins C and K3 to play an adjuvant role in the treatment of ovarian cancer.
Original language | English |
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Pages (from-to) | 3279-3287 |
Number of pages | 9 |
Journal | Anticancer Research |
Volume | 23 |
Issue number | 4 |
Publication status | Published - Jul 2003 |
Externally published | Yes |
Keywords
- Autoschizis
- Ovarian cancer
- Vitamin C
- Vitamin K