The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation

Marino Caruso, Sébastien Meurant, Damien Detraux, Amandine Mathieu, Manon Gilson, Marc Dieu, Antoine Fattaccioli, Catherine Demazy, Mustapha Najimi, Etienne Sokal, Thierry Arnould, Catherine Verfaillie, Denis L.J. Lafontaine, Patricia Renard

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Abstract

Translational regulation is of paramount importance for proteome remodeling during stem cell differentiation at both the global and the transcript-specific levels. In this study, we characterized translational remodeling during hepatogenic differentiation of induced pluripotent stem cells (iPSCs) by polysome profiling. We demonstrate that protein synthesis increases during exit from pluripotency and is then globally repressed during later steps of hepatogenic maturation. This global downregulation of translation is accompanied by a decrease in the abundance of protein components of the translation machinery, which involves a global reduction in translational efficiency of terminal oligopyrimidine tract (TOP) mRNA encoding translation-related factors. Despite global translational repression during hepatogenic differentiation, key hepatogenic genes remain efficiently translated, and the translation of several transcripts involved in hepatospecific functions and metabolic maturation is even induced. We conclude that, during hepatogenic differentiation, a global decrease in protein synthesis is accompanied by a specific translational rewiring of hepatospecific transcripts.

Original languageEnglish
Pages (from-to)254-268
Number of pages15
JournalStem Cell Reports
Volume18
Issue number1
DOIs
Publication statusPublished - 10 Jan 2023

Keywords

  • hepatocyte differentiation
  • iPSC
  • LARP1
  • polysome profiling
  • stem cells
  • TOP mRNA
  • translational regulation

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