Target immunity of the Tn3-family transposon Tn4430 requires specific interactions between the transposase and the terminal inverted repeats of the transposon

Emilien Nicolas; Michaël Lambin; Bernard Hallet

doi:10.1128/JB.00477-10

Target immunity of the Tn3-family transposon Tn4430 requires specific interactions between the transposase and the terminal inverted repeats of the transposon

Emilien Nicolas, Michaël Lambin, Bernard Hallet

Research output: Contribution to journal › Article › peer-review

Abstract

Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner.

Original language	English
Pages (from-to)	4233-8
Number of pages	6
Journal	Journal of Bacteriology
Volume	192
Issue number	16
DOIs	https://doi.org/10.1128/JB.00477-10
Publication status	Published - 2010
Externally published	Yes

Keywords

DNA Transposable Elements
DNA, Bacterial/metabolism
Escherichia coli/genetics
Protein Binding
Recombinant Fusion Proteins/genetics
Repressor Proteins/genetics
Terminal Repeat Sequences
Transposases/genetics

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10.1128/JB.00477-10

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title = "Target immunity of the Tn3-family transposon Tn4430 requires specific interactions between the transposase and the terminal inverted repeats of the transposon",

abstract = "Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner.",

keywords = "DNA Transposable Elements, DNA, Bacterial/metabolism, Escherichia coli/genetics, Protein Binding, Recombinant Fusion Proteins/genetics, Repressor Proteins/genetics, Terminal Repeat Sequences, Transposases/genetics",

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year = "2010",

doi = "10.1128/JB.00477-10",

language = "English",

volume = "192",

pages = "4233--8",

journal = "Journal of Bacteriology",

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publisher = "American Society for Microbiology",

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T1 - Target immunity of the Tn3-family transposon Tn4430 requires specific interactions between the transposase and the terminal inverted repeats of the transposon

AU - Nicolas, Emilien

AU - Lambin, Michaël

AU - Hallet, Bernard

PY - 2010

Y1 - 2010

N2 - Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner.

AB - Specificity of the Tn4430 target immunity signal was examined by fusing the transposase TnpA to the LacI repressor of Escherichia coli. The resulting chimeric proteins failed to impose immunity to DNA targets carrying copies of the lacO operator, though they were proficient in lacO binding in vivo and remained responsive to wild-type immunity conferred by the Tn4430 inverted repeat end. Intriguingly, the presence of lacO repeats within the target was found to strongly influence target site selection by Tn4430, but in a LacI-independent manner.

KW - DNA Transposable Elements

KW - DNA, Bacterial/metabolism

KW - Escherichia coli/genetics

KW - Protein Binding

KW - Recombinant Fusion Proteins/genetics

KW - Repressor Proteins/genetics

KW - Terminal Repeat Sequences

KW - Transposases/genetics

U2 - 10.1128/JB.00477-10

DO - 10.1128/JB.00477-10

M3 - Article

C2 - 20562304

SN - 0021-9193

VL - 192

SP - 4233

EP - 4238

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 16

ER -

Target immunity of the Tn3-family transposon Tn4430 requires specific interactions between the transposase and the terminal inverted repeats of the transposon

Abstract

Keywords

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