Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway

Richard Raj Singh, Buncha Chinnasri, Lien De Smet, Ashley Haeck, Kristof Demeestere, Pierre Van Cutsem, Geraldine Van Aubel, Godelieve Gheysen, Tina Kyndt

Research output: Contribution to journalArticle

Abstract

Activation of induced plant resistance to control pests and diseases is regaining attention in the current climate where chemical pesticides are being progressively banned. Formulations of chitosan oligomers (COS) and pectin-derived oligogalacturonides (OGA), COS-OGA, have previously been described to induce resistance against fungal diseases in different crop plants. Here, we investigated their potential and mode-of-action as preventive measures to control root-knot nematode Meloidogyne graminicola infection in rice. The results show a significant reduction in root-galling and nematode development in rice plants that were treated through foliar application with the COS-OGA formulations FytoSol® and FytoSave® 24 h before nematode inoculation. Hormone measurements, gene expression analyses, corroborated by treatments on salicylic acid (SA) and jasmonic acid (JA)-mutants indicated that the systemic COS-OGA induced defense mechanism against nematodes is not based on SA or JA activation. However, phenylalanine ammonia lyase (PAL) gene expression in roots as well as enzymatic PAL activity in the shoots were significantly induced 24 h after foliar COS-OGA spraying in comparison with untreated plants. COS-OGA-induced systemic defense was abolished in the rice OsPAL4-mutant, demonstrating that COS-OGA-induced defense is dependent on OsPAL4 activation in rice plants.
Original languageEnglish
Pages (from-to)202-210
Number of pages9
JournalPlant Physiology and Biochemistry
Volume142
DOIs
Publication statusPublished - 1 Sep 2019

Fingerprint

Chitosan
phenylpropanoids
root-knot nematodes
chitosan
rice
Phenylalanine Ammonia-Lyase
Salicylic Acid
Nematoda
phenylalanine ammonia-lyase
jasmonic acid
salicylic acid
Meloidogyne graminicola
Tylenchoidea
Gene Expression
gene expression
mutants
Pest Control
Mycoses
foliar application
Oryza

Keywords

  • Hormone
  • Phenylpropanoids
  • Plant defense elicitor

Cite this

Singh, Richard Raj ; Chinnasri, Buncha ; De Smet, Lien ; Haeck, Ashley ; Demeestere, Kristof ; Van Cutsem, Pierre ; Van Aubel, Geraldine ; Gheysen, Godelieve ; Kyndt, Tina. / Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway. In: Plant Physiology and Biochemistry. 2019 ; Vol. 142. pp. 202-210.
@article{81627be3f05744489686268cfa0aebc9,
title = "Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway",
abstract = "Activation of induced plant resistance to control pests and diseases is regaining attention in the current climate where chemical pesticides are being progressively banned. Formulations of chitosan oligomers (COS) and pectin-derived oligogalacturonides (OGA), COS-OGA, have previously been described to induce resistance against fungal diseases in different crop plants. Here, we investigated their potential and mode-of-action as preventive measures to control root-knot nematode Meloidogyne graminicola infection in rice. The results show a significant reduction in root-galling and nematode development in rice plants that were treated through foliar application with the COS-OGA formulations FytoSol{\circledR} and FytoSave{\circledR} 24 h before nematode inoculation. Hormone measurements, gene expression analyses, corroborated by treatments on salicylic acid (SA) and jasmonic acid (JA)-mutants indicated that the systemic COS-OGA induced defense mechanism against nematodes is not based on SA or JA activation. However, phenylalanine ammonia lyase (PAL) gene expression in roots as well as enzymatic PAL activity in the shoots were significantly induced 24 h after foliar COS-OGA spraying in comparison with untreated plants. COS-OGA-induced systemic defense was abolished in the rice OsPAL4-mutant, demonstrating that COS-OGA-induced defense is dependent on OsPAL4 activation in rice plants.",
keywords = "Hormone, Phenylpropanoids, Plant defense elicitor",
author = "Singh, {Richard Raj} and Buncha Chinnasri and {De Smet}, Lien and Ashley Haeck and Kristof Demeestere and {Van Cutsem}, Pierre and {Van Aubel}, Geraldine and Godelieve Gheysen and Tina Kyndt",
year = "2019",
month = "9",
day = "1",
doi = "10.1016/j.plaphy.2019.07.003",
language = "English",
volume = "142",
pages = "202--210",
journal = "Plant Physiology and Biochemistry",
issn = "0981-9428",
publisher = "Elsevier Masson SAS",

}

Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway. / Singh, Richard Raj; Chinnasri, Buncha; De Smet, Lien; Haeck, Ashley; Demeestere, Kristof; Van Cutsem, Pierre; Van Aubel, Geraldine; Gheysen, Godelieve; Kyndt, Tina.

In: Plant Physiology and Biochemistry, Vol. 142, 01.09.2019, p. 202-210.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Systemic defense activation by COS-OGA in rice against root-knot nematodes depends on stimulation of the phenylpropanoid pathway

AU - Singh, Richard Raj

AU - Chinnasri, Buncha

AU - De Smet, Lien

AU - Haeck, Ashley

AU - Demeestere, Kristof

AU - Van Cutsem, Pierre

AU - Van Aubel, Geraldine

AU - Gheysen, Godelieve

AU - Kyndt, Tina

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Activation of induced plant resistance to control pests and diseases is regaining attention in the current climate where chemical pesticides are being progressively banned. Formulations of chitosan oligomers (COS) and pectin-derived oligogalacturonides (OGA), COS-OGA, have previously been described to induce resistance against fungal diseases in different crop plants. Here, we investigated their potential and mode-of-action as preventive measures to control root-knot nematode Meloidogyne graminicola infection in rice. The results show a significant reduction in root-galling and nematode development in rice plants that were treated through foliar application with the COS-OGA formulations FytoSol® and FytoSave® 24 h before nematode inoculation. Hormone measurements, gene expression analyses, corroborated by treatments on salicylic acid (SA) and jasmonic acid (JA)-mutants indicated that the systemic COS-OGA induced defense mechanism against nematodes is not based on SA or JA activation. However, phenylalanine ammonia lyase (PAL) gene expression in roots as well as enzymatic PAL activity in the shoots were significantly induced 24 h after foliar COS-OGA spraying in comparison with untreated plants. COS-OGA-induced systemic defense was abolished in the rice OsPAL4-mutant, demonstrating that COS-OGA-induced defense is dependent on OsPAL4 activation in rice plants.

AB - Activation of induced plant resistance to control pests and diseases is regaining attention in the current climate where chemical pesticides are being progressively banned. Formulations of chitosan oligomers (COS) and pectin-derived oligogalacturonides (OGA), COS-OGA, have previously been described to induce resistance against fungal diseases in different crop plants. Here, we investigated their potential and mode-of-action as preventive measures to control root-knot nematode Meloidogyne graminicola infection in rice. The results show a significant reduction in root-galling and nematode development in rice plants that were treated through foliar application with the COS-OGA formulations FytoSol® and FytoSave® 24 h before nematode inoculation. Hormone measurements, gene expression analyses, corroborated by treatments on salicylic acid (SA) and jasmonic acid (JA)-mutants indicated that the systemic COS-OGA induced defense mechanism against nematodes is not based on SA or JA activation. However, phenylalanine ammonia lyase (PAL) gene expression in roots as well as enzymatic PAL activity in the shoots were significantly induced 24 h after foliar COS-OGA spraying in comparison with untreated plants. COS-OGA-induced systemic defense was abolished in the rice OsPAL4-mutant, demonstrating that COS-OGA-induced defense is dependent on OsPAL4 activation in rice plants.

KW - Hormone

KW - Phenylpropanoids

KW - Plant defense elicitor

UR - http://www.scopus.com/inward/record.url?scp=85068552849&partnerID=8YFLogxK

U2 - 10.1016/j.plaphy.2019.07.003

DO - 10.1016/j.plaphy.2019.07.003

M3 - Article

VL - 142

SP - 202

EP - 210

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

ER -