Surface properties and cell adhesion onto allylamine-plasma and amine-plasma coated glass coverslips

Marianne Crespin, Nicolas Moreau, Bernard Masereel, Olivier Feron, Bernard Gallez, Thierry Vander Borght, Carine Michiels, Stephane Lucas

Research output: Contribution to journalArticle

Abstract

Surface properties of nanoparticles to be used for radioimmunotherapy need to be optimized to allow antibody conjugation while ensuring biocompatibility. We aimed to investigate cell adhesion and proliferation onto different coatings to be used for nanoparticles. C, CH or SiO coatings deposited onto glass coverslips by magnetron deposition as well as nitrogen functionalized materials synthetized using different reactive sputtering conditions and PPAA (plasma polymerized allylamine) coating, were compared. Amine functionalization did increase hydrophilicity in all the materials tested. Biocompatibility was assessed by measuring cell viability, morphology, attachment, spreading, and pro-inflammatory cytokine secretion. The results show that C and CN were the most biocompatible substrates while SiO and SiO N were the most toxic materials. PPAA coatings displayed unexpectedly an intermediate biocompatibility. A correlation could be observed between wettability and cell proliferation except for C coated surface, indicating that more complex processes than hydrophilicity alone are taking place that affect cell functions. © Springer Science+Business Media, LLC 2011.
Original languageEnglish
Pages (from-to)671-682
Number of pages12
JournalJournal of Materials Science: Materials in Medicine
Volume22
Issue number3
DOIs
Publication statusPublished - Mar 2011

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Keywords

  • Allylamine
  • Amines
  • Antibodies
  • Biocompatible Materials
  • Cell Adhesion
  • Cell Survival
  • Coated Materials, Biocompatible
  • Cytokines
  • Endothelial Cells
  • Glass
  • Humans
  • Inflammation
  • Nanoparticles
  • Nanotechnology
  • Radioimmunotherapy
  • Surface Properties

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