Sum-Frequency Generation Spectroscopy of DNA Monolayers

Yannick Sartenaer, Gerard Tourillon, Laurent Dreesen, Dan Lis, Alaa Addin Mani, Paul Thiry, André Peremans

Research output: Contribution to journalArticle

Abstract

The anchoring of thiolated single-stranded DNA (HS-ssDNA) monolayers onto platinum substrates was investigated by Sum-Frequency Generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700-3100 cm-1 spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH2PO4/K2HPO4/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on Platinum.
Original languageEnglish
Pages (from-to)2179-2183
JournalBiosensors and Bioelectronics
Volume22
Publication statusPublished - 2007

Fingerprint

Platinum
Monolayers
Spectrum Analysis
Buffers
DNA
Spectroscopy
Self assembled monolayers
Tromethamine
Single-Stranded DNA
Dermatoglyphics
Edetic Acid
Molecules
Ethylenediaminetetraacetic acid
Substrates
Intercalation
Anchors
Water
potassium phosphate

Cite this

Sartenaer, Y., Tourillon, G., Dreesen, L., Lis, D., Mani, A. A., Thiry, P., & Peremans, A. (2007). Sum-Frequency Generation Spectroscopy of DNA Monolayers. Biosensors and Bioelectronics, 22, 2179-2183.
Sartenaer, Yannick ; Tourillon, Gerard ; Dreesen, Laurent ; Lis, Dan ; Mani, Alaa Addin ; Thiry, Paul ; Peremans, André. / Sum-Frequency Generation Spectroscopy of DNA Monolayers. In: Biosensors and Bioelectronics. 2007 ; Vol. 22. pp. 2179-2183.
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Sartenaer, Y, Tourillon, G, Dreesen, L, Lis, D, Mani, AA, Thiry, P & Peremans, A 2007, 'Sum-Frequency Generation Spectroscopy of DNA Monolayers', Biosensors and Bioelectronics, vol. 22, pp. 2179-2183.

Sum-Frequency Generation Spectroscopy of DNA Monolayers. / Sartenaer, Yannick; Tourillon, Gerard; Dreesen, Laurent; Lis, Dan; Mani, Alaa Addin; Thiry, Paul; Peremans, André.

In: Biosensors and Bioelectronics, Vol. 22, 2007, p. 2179-2183.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sum-Frequency Generation Spectroscopy of DNA Monolayers

AU - Sartenaer, Yannick

AU - Tourillon, Gerard

AU - Dreesen, Laurent

AU - Lis, Dan

AU - Mani, Alaa Addin

AU - Thiry, Paul

AU - Peremans, André

PY - 2007

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AB - The anchoring of thiolated single-stranded DNA (HS-ssDNA) monolayers onto platinum substrates was investigated by Sum-Frequency Generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700-3100 cm-1 spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH2PO4/K2HPO4/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on Platinum.

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JO - Biosensors and Bioelectronics

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Sartenaer Y, Tourillon G, Dreesen L, Lis D, Mani AA, Thiry P et al. Sum-Frequency Generation Spectroscopy of DNA Monolayers. Biosensors and Bioelectronics. 2007;22:2179-2183.