Sum-Frequency Generation Spectroscopy of DNA Monolayers

Yannick Sartenaer, Gerard Tourillon, Laurent Dreesen, Dan Lis, Alaa Addin Mani, Paul Thiry, André Peremans

Research output: Contribution to journalArticle


The anchoring of thiolated single-stranded DNA (HS-ssDNA) monolayers onto platinum substrates was investigated by Sum-Frequency Generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700-3100 cm-1 spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH2PO4/K2HPO4/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on Platinum.
Original languageEnglish
Pages (from-to)2179-2183
JournalBiosensors and Bioelectronics
Publication statusPublished - 2007


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