Sum-frequency generation spectroscopy applied to model biosensors systems

Laurent Dreesen, Yannick Sartenaer, Christophe Humbert, Alaa Addin Mani, Jean-Jacques Lemaire, Christophe Méthivier, Claire-Marie Pradier, Paul Thiry, André Peremans

Research output: Contribution to journalArticle

Abstract

Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin.
Original languageEnglish
Pages (from-to)373-378
Number of pages6
JournalThin Solid Films
Volume464-465
Publication statusPublished - 2004

Fingerprint

bioinstrumentation
Biosensors
Avidin
Spectroscopy
Substrates
spectroscopy
vitamins
Vitamins
methylidyne
Infrared radiation
proteins
Proteins
Adsorption
vibration
adsorption
configurations
Experiments
biocytin

Cite this

Dreesen, L., Sartenaer, Y., Humbert, C., Mani, A. A., Lemaire, J-J., Méthivier, C., ... Peremans, A. (2004). Sum-frequency generation spectroscopy applied to model biosensors systems. Thin Solid Films, 464-465, 373-378.
Dreesen, Laurent ; Sartenaer, Yannick ; Humbert, Christophe ; Mani, Alaa Addin ; Lemaire, Jean-Jacques ; Méthivier, Christophe ; Pradier, Claire-Marie ; Thiry, Paul ; Peremans, André. / Sum-frequency generation spectroscopy applied to model biosensors systems. In: Thin Solid Films. 2004 ; Vol. 464-465. pp. 373-378.
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Dreesen, L, Sartenaer, Y, Humbert, C, Mani, AA, Lemaire, J-J, Méthivier, C, Pradier, C-M, Thiry, P & Peremans, A 2004, 'Sum-frequency generation spectroscopy applied to model biosensors systems', Thin Solid Films, vol. 464-465, pp. 373-378.

Sum-frequency generation spectroscopy applied to model biosensors systems. / Dreesen, Laurent; Sartenaer, Yannick; Humbert, Christophe; Mani, Alaa Addin; Lemaire, Jean-Jacques; Méthivier, Christophe; Pradier, Claire-Marie; Thiry, Paul; Peremans, André.

In: Thin Solid Films, Vol. 464-465, 2004, p. 373-378.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sum-frequency generation spectroscopy applied to model biosensors systems

AU - Dreesen, Laurent

AU - Sartenaer, Yannick

AU - Humbert, Christophe

AU - Mani, Alaa Addin

AU - Lemaire, Jean-Jacques

AU - Méthivier, Christophe

AU - Pradier, Claire-Marie

AU - Thiry, Paul

AU - Peremans, André

PY - 2004

Y1 - 2004

N2 - Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin.

AB - Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin.

M3 - Article

VL - 464-465

SP - 373

EP - 378

JO - Thin Solid Films

JF - Thin Solid Films

SN - 0040-6090

ER -

Dreesen L, Sartenaer Y, Humbert C, Mani AA, Lemaire J-J, Méthivier C et al. Sum-frequency generation spectroscopy applied to model biosensors systems. Thin Solid Films. 2004;464-465:373-378.