Abstract
Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a
derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin.
Original language | English |
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Pages (from-to) | 373-378 |
Number of pages | 6 |
Journal | Thin Solid Films |
Volume | 464-465 |
Publication status | Published - 2004 |