Study by the sum-frequency generation spectroscopy of a model biosensor : The biocytin-avidin complex

Yannick Sartenaer, Laurent Dreesen, Christophe Humbert, Alaa Addin Mani, Jean-Jacques Lemaire, Michelle Salmain, Claire-Marie Pradier, Paul Thiry, André Peremans

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Abstract

Infrared-visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derivated vitamin (biocytin). No interaction is observed when the measurements are performed on metallic substrates in the normal co-propagative configuration. When the biocytin layer is deposited on a CaF2 substrate and the SFG measurements are performed in the total internal reflection configuration, drastic modifications in the C-H and N-H spectral ranges attest the molecular recognition of avidin by biocytin. The specificity of the recognition process is confirmed by replacing avidin by other proteins.
Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalPhysicalia Magazine
Volume25
Issue number3
Publication statusPublished - 2003

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