Abstract
Infrared-visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derivated vitamin (biocytin). No interaction is observed when the measurements are performed on metallic substrates in the normal co-propagative configuration. When the biocytin layer is deposited on a CaF2 substrate and the SFG measurements are performed in the total internal reflection configuration, drastic modifications in the C-H and N-H spectral ranges attest the molecular recognition of avidin by biocytin. The specificity of the recognition process is confirmed by replacing avidin by other proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 221-229 |
| Number of pages | 9 |
| Journal | Physicalia Magazine |
| Volume | 25 |
| Issue number | 3 |
| Publication status | Published - 2003 |