Projects per year
Structural changes of solid-supported lipid bilayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and of their interfacial water surrounding have been probed with vibrational sum frequency generation (SFG) upon interaction with functionalized gold NPs, carrying negative or positive surface charges. Switching the substrate/DPPC zeta potential from strongly negative to weakly negative or positive values by using SiO2 or CaF2 supporting surfaces has enabled elucidating interfacial charge effects, intermolecular interactions, and interaction mechanisms regulating those nano-bio interfaces. At SiO2/DPPC surfaces (ζ = -30 mV), negative NPs have reinforced the average interfacial water alignment, while no interaction has occurred with DPPC bilayer. Oppositely, positive NPs interact with DPPC, with a probable two-step mechanism involving the formation of pores within the bilayer followed by a reorganization of a quasi-ordered DPPC bilayer around NPs. At CaF2/DPPC surfaces (ζ = ±15 mV), both negative and positive NPs have clearly damaged the lipid bilayer structure, together with a drastic disruption of the water organization at the interface. Importantly, all major interfacial structural changes have occurred within the first 15-90 min after exposure to NPs whatever the lipid is supported on SiO2 or CaF2.
CECCHET, F. & TOLEDO-FUENTES, X.
1/10/11 → …
Action Nationale de Formation du CNRS en "Spectroscopie et Imagerie d’interfaces materiaux- molecules du vivant"
Francesca Cecchet (Invited Speaker)15 Oct 2014 → 17 Oct 2014
Activity: Participating in or organising an event types › Participation in conference
Study of the interactions between gold nanoparticles and models of cell membranes by second order nonlinear optical spectroscopiesAuthor: Toledo-Fuentes, X., 28 Oct 2015
Student thesis: Doc types › Doctor of Sciences