Strong effects of amoebae grazing on the biomass and genetic structure of a Microcystis bloom (Cyanobacteria)

J. Van Wichelen, I. van Gremberghe, P. Vanormelingen, A.-E. Debeer, W. Vyverman, B. Leporcq, J.-P. Descy, D. Menzel, G.A. Codd

Research output: Contribution to journalArticle

Abstract

Despite its importance for bloom toxicity, the factors determining the population structure of cyanobacterial blooms are poorly understood. Here, we report the results of a two-year field survey of the population dynamics of Microcystis blooms in a small hypertrophic urban pond. Microscopic enumeration of Microcystis and its predators and parasites was combined with pigment and microcystin analysis and denaturing gradient gel electrophoresis of the ITS rDNA region to assess population dynamics and structure. Two main Microcystis morpho- and ITS types were revealed, corresponding to M. aeruginosa and M. viridis. In both years, high population densities of naked amoebae grazing on Microcystis coincided with rapid decreases in Microcystis biomass. In one year, there was a shift from heavily infested M. aeruginosa to the less-infested M. viridis, allowing the bloom to rapidly recover. The preference of amoebae for M. aeruginosa was confirmed by grazing experiments, in which several amoeba strains were capable of grazing down a strain of M. aeruginosa, but not of M. viridis. Zooplankton and chytrid parasites appeared to be of minor importance for these strong and fast reductions in Microcystis biomass. These findings demonstrate a strong impact of small protozoan grazers on the biomass and genetic structure of Microcystis blooms.
Original languageEnglish
Pages (from-to)2797-2813
Number of pages17
JournalApplied and Environmental Microbiology
Volume12
Issue number10
DOIs
Publication statusPublished - 1 Oct 2010

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Microcystis
Amoeba
Genetic Structures
Cyanobacteria
Biomass
genetic structure
cyanobacterium
algal bloom
grazing
biomass
population structure
Microcystis aeruginosa
population dynamics
parasite
Population Dynamics
Parasites
Zooplankton
parasites
field survey
Denaturing Gradient Gel Electrophoresis

Cite this

Van Wichelen, J. ; van Gremberghe, I. ; Vanormelingen, P. ; Debeer, A.-E. ; Vyverman, W. ; Leporcq, B. ; Descy, J.-P. ; Menzel, D. ; Codd, G.A. / Strong effects of amoebae grazing on the biomass and genetic structure of a Microcystis bloom (Cyanobacteria). In: Applied and Environmental Microbiology. 2010 ; Vol. 12, No. 10. pp. 2797-2813.
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Strong effects of amoebae grazing on the biomass and genetic structure of a Microcystis bloom (Cyanobacteria). / Van Wichelen, J.; van Gremberghe, I.; Vanormelingen, P.; Debeer, A.-E.; Vyverman, W.; Leporcq, B.; Descy, J.-P.; Menzel, D.; Codd, G.A.

In: Applied and Environmental Microbiology, Vol. 12, No. 10, 01.10.2010, p. 2797-2813.

Research output: Contribution to journalArticle

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