Abstract
To test the involvement of the telomeres in the senescent phenotype, we used telomerase-immortalized human foreskin fibroblasts (hTERT-BJ1). We exposed hTERT-BJ1 and parental BJ cells to either UVB or H2O2 subcytotoxic stress(es). Both cell lines developed biomarkers of replicative senescence: loss of replicative potential, increase in senescence-associated β-galactosidase activity, typical senescence-like morphology, overexpression of p21WAF-1 and p16INK-4a, and decreased level of the hyperphosphorylated form of pRb. Telomere shortening was slightly higher under stress for both BJ and hTERT-BJ1 but still much lower than that reported for other cell lines. We conclude that pathways alternative to telomere shortening must cause the appearance of the senescence phenotype. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 157-162 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 523 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 17 Jul 2002 |
Keywords
- Cellular senescence
- Fibroblast
- HO
- Telomerase
- Telomere
- UVB