Spleen-dependent turnover of CD11b peripheral blood B lymphocytes in bovine leukemia virus-infected sheep.

A.-F. Florins, N. Gillet, B. Asquith, Christophe DEBACQ, Grégori Jean, I. Schwartz-Cornil, M. Bonneau, Arsène Burny, M. Reichert, R Kettmann, L. Willems

Research output: Contribution to journalArticlepeer-review

Abstract

[en] Lymphocyte homeostasis is determined by a critical balance between cell proliferation and death, an equilibrium which is deregulated in bovine leukemia virus (BLV)-infected sheep. We have previously shown that an excess of proliferation occurs in lymphoid tissues and that the peripheral blood population is prone to increased cell death. To further understand the mechanisms involved, we evaluated the physiological role of the spleen in this accelerated turnover. To this end, B lymphocytes were labeled in vivo using a fluorescent marker (carboxyfluorescein diacetate succinimidyl ester), and the cell kinetic parameters (proliferation and death rates) of animals before and after splenectomy were compared. We show that the enhanced cell death observed in BLV-infected sheep is abrogated after splenectomy, revealing a key role of the spleen in B-lymphocyte dynamics.
Original languageEnglish
JournalJournal of Virology
Volume80
Issue number24
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Sciences du vivant => Médecine vétérinaire santé animale
  • Sciences de la santé humaine => Oncologie
  • Animals
  • Antigens, CD11b/immunology
  • Apoptosis/immunology
  • B-Lymphocytes/immunology
  • Cell Proliferation
  • DNA Primers
  • Deltaretrovirus Infections/immunology
  • Fluoresceins
  • Homeostasis/immunology
  • Immunophenotyping
  • Kinetics
  • Leukemia Virus, Bovine/immunology
  • Sheep
  • Spleen/immunology
  • Splenectomy
  • Succinimides

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