Soluble factors released by virus specific activated cytotoxic T-lymphocytes induce apoptotic death of astroglioma cell lines

Farideh Sabri, Francesca Chiodi, Jean-Pascal Piret, Cheng-Hong Wei, Eugene Major, Bengt Westermark, Maria G Masucci, Victor Levitsky

Research output: Contribution to journalArticle

Abstract

Astrocytomas and astrogliomas represent the most common types of primary tumors in human central nervous system and are associated with high mortality due to the absence of efficient therapy. Here we demonstrate that, upon antigen-specific activation, cytotoxic T-lymphocytes (CTLs) secrete products that inhibit proliferation and induce apoptosis in a significant proportion of astroglioma cell lines. This effect is tumor specific in that normal cultured astrocytes do not develop apoptotic changes upon exposure to supernatant of activated CTLs. Experiments with purified lymphokines and lymphokine specific blocking antibodies indicate that synergistic activities of tumor necrosis factor (TNF)-alpha and interferon (INF)-gamma are required for the apoptosis inducing effect on some astroglioma cell lines. However, this effect appears to be dependent on additional factors produced by activated CTLs. Our results suggest that local application of factors released by activated CTLs or induction of CTL migration and activation in the tumor site may have a therapeutic effect in patients with astrogliomas.
Original languageEnglish
Pages (from-to)165-75
Number of pages11
JournalBrain pathology (Zurich, Switzerland)
Volume13
Issue number2
Publication statusPublished - Apr 2003

Keywords

  • Animals
  • Annexin A5
  • Apoptosis
  • Astrocytoma
  • Brain Neoplasms
  • Cell Transformation, Viral
  • Cells, Cultured
  • Humans
  • Lymphocyte Activation
  • Lymphokines
  • Mice
  • T-Lymphocytes, Cytotoxic
  • Tumor Cells, Cultured

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    Sabri, F., Chiodi, F., Piret, J-P., Wei, C-H., Major, E., Westermark, B., Masucci, M. G., & Levitsky, V. (2003). Soluble factors released by virus specific activated cytotoxic T-lymphocytes induce apoptotic death of astroglioma cell lines. Brain pathology (Zurich, Switzerland), 13(2), 165-75.