Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions

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Abstract

Chitosan oligomers are known elicitors of plant defence mechanisms. In this work, chitooligosaccharides of different degrees of polymerization and degrees of acetylation were prepared and characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The effect of the degree of polymerization (DP), degree of acetylation and concentration of these chitooligosaccharides on defence activation in Arabidopsis thaliana suspension-cultured cells was studied. Our study results show that fully deacetylated chitooligosaccharides (chitosan oligomers) induce, depending on their DP and concentration, phenylalanine ammonia-lyase (PAL) activation, HO synthesis and cell death in A. thaliana cell suspensions. The progressive reacetylation of the chitosan oligomer elicitors progressively impaired their ability to enhance HO accumulation and cell death, but did not affect the activation of PAL.
Original languageEnglish
Pages (from-to)44-56
Number of pages13
JournalPHYSIOLOGIA PLANTARUM
Volume127
Issue number1
DOIs
Publication statusPublished - 1 May 2006

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chitooligosaccharides
Phenylalanine Ammonia-Lyase
Peroxides
Chitosan
acetylation
phenylalanine ammonia-lyase
Acetylation
chitosan
peroxides
Arabidopsis
Polymerization
polymerization
cell suspension culture
cell death
Suspensions
Cell Death
Water
Arabidopsis thaliana
matrix-assisted laser desorption-ionization mass spectrometry
water

Cite this

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title = "Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions",
abstract = "Chitosan oligomers are known elicitors of plant defence mechanisms. In this work, chitooligosaccharides of different degrees of polymerization and degrees of acetylation were prepared and characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The effect of the degree of polymerization (DP), degree of acetylation and concentration of these chitooligosaccharides on defence activation in Arabidopsis thaliana suspension-cultured cells was studied. Our study results show that fully deacetylated chitooligosaccharides (chitosan oligomers) induce, depending on their DP and concentration, phenylalanine ammonia-lyase (PAL) activation, HO synthesis and cell death in A. thaliana cell suspensions. The progressive reacetylation of the chitosan oligomer elicitors progressively impaired their ability to enhance HO accumulation and cell death, but did not affect the activation of PAL.",
author = "{Cabrera Pino}, {Juan Carlos} and J. Messiaen and P. Cambier and {Van Cutsem}, P.",
year = "2006",
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doi = "10.1111/j.1399-3054.2006.00677.x",
language = "English",
volume = "127",
pages = "44--56",
journal = "PHYSIOLOGIA PLANTARUM",
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TY - JOUR

T1 - Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions

AU - Cabrera Pino, Juan Carlos

AU - Messiaen, J.

AU - Cambier, P.

AU - Van Cutsem, P.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Chitosan oligomers are known elicitors of plant defence mechanisms. In this work, chitooligosaccharides of different degrees of polymerization and degrees of acetylation were prepared and characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The effect of the degree of polymerization (DP), degree of acetylation and concentration of these chitooligosaccharides on defence activation in Arabidopsis thaliana suspension-cultured cells was studied. Our study results show that fully deacetylated chitooligosaccharides (chitosan oligomers) induce, depending on their DP and concentration, phenylalanine ammonia-lyase (PAL) activation, HO synthesis and cell death in A. thaliana cell suspensions. The progressive reacetylation of the chitosan oligomer elicitors progressively impaired their ability to enhance HO accumulation and cell death, but did not affect the activation of PAL.

AB - Chitosan oligomers are known elicitors of plant defence mechanisms. In this work, chitooligosaccharides of different degrees of polymerization and degrees of acetylation were prepared and characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The effect of the degree of polymerization (DP), degree of acetylation and concentration of these chitooligosaccharides on defence activation in Arabidopsis thaliana suspension-cultured cells was studied. Our study results show that fully deacetylated chitooligosaccharides (chitosan oligomers) induce, depending on their DP and concentration, phenylalanine ammonia-lyase (PAL) activation, HO synthesis and cell death in A. thaliana cell suspensions. The progressive reacetylation of the chitosan oligomer elicitors progressively impaired their ability to enhance HO accumulation and cell death, but did not affect the activation of PAL.

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