Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

Clémence Whiteway; Adam Valcek; Chantal Philippe; Mojca Strazisar; Tim De Pooter; Ivan Mateus; Anke Breine; Charles Van der Henst

doi:10.1038/s41396-021-01179-3

Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

Clémence Whiteway, Adam Valcek, Chantal Philippe, Mojca Strazisar, Tim De Pooter, Ivan Mateus, Anke Breine, Charles Van der Henst

Research Unit in Biology of Microorganisms

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Abstract

We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.

Original language	English
Pages (from-to)	1473-1477
Number of pages	5
Journal	ISME Journal
Volume	16
Issue number	5
DOIs	https://doi.org/10.1038/s41396-021-01179-3
Publication status	Published - May 2022

Funding

This project was supported by the Flanders Institute for Biotechnology (VIB) and has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 748032 (CV). AB is recipient of a PhD fellowship Strategic Basic Research of the Research Foundation – Flanders (FWO, File number: 77258).

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s41396-021-01179-3Final published version, 1.19 MBLicence: CC BY

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title = "Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii",

abstract = "We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.",

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doi = "10.1038/s41396-021-01179-3",

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T1 - Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

AU - Whiteway, Clémence

AU - Valcek, Adam

AU - Philippe, Chantal

AU - Strazisar, Mojca

AU - De Pooter, Tim

AU - Mateus, Ivan

AU - Breine, Anke

AU - Van der Henst, Charles

N1 - Funding Information: This project was supported by the Flanders Institute for Biotechnology (VIB) and has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 748032 (CV). AB is recipient of a PhD fellowship Strategic Basic Research of the Research Foundation – Flanders (FWO, File number: 77258). Publisher Copyright: © 2022, The Author(s).

PY - 2022/5

Y1 - 2022/5

N2 - We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.

AB - We identify a new mechanism mediating capsule production and virulence in the WHO and CDC priority ESKAPE pathogen Acinetobacter baumannii. Non-capsulated and avirulent bacteria can revert into a capsulated and virulent state upon scarless excision of an ISAba13 insertion sequence under stress conditions. Reversion events fully restore capsule production and in vivo virulence. This increases our knowledge about A. baumannii genome dynamics, and the regulation of capsule production, virulence and resistance.

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Scarless excision of an insertion sequence restores capsule production and virulence in Acinetobacter baumannii

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