SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab

Damien Coupeau; Nicolas Burton; Noémie Lejeune; Suzanne Loret; Astrid Petit; Srđan Pejaković; Florian Poulain; Laura Bonil; Gabrielle Trozzi; Laetitia Wiggers; Kévin Willemart; Emmanuel André; Lies Laenen; Lize Cuypers; Marc Van Ranst; Pierre Bogaerts; Benoît Muylkens; nicolas gillet

doi:10.3390/mps3030059

SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab

Damien Coupeau, Nicolas Burton, Noémie Lejeune, Suzanne Loret, Astrid Petit, Srđan Pejaković, Florian Poulain, Laura Bonil, Gabrielle Trozzi, Laetitia Wiggers, Kévin Willemart, Emmanuel André, Lies Laenen, Lize Cuypers, Marc Van Ranst, Pierre Bogaerts, Benoît Muylkens, nicolas gillet

Research output: Contribution to journal › Article › peer-review

Abstract

The emergence of the SARS-CoV-2 virus and the exponential growth of COVID-19 cases have created a major crisis for public health systems. The critical identification of contagious asymptomatic carriers requires the isolation of viral nucleic acids, reverse transcription, and amplification by PCR. However, the shortage of specific proprietary reagents or the lack of automated platforms have seriously hampered diagnostic throughput in many countries. Here, we provide a procedure for SARS-CoV-2 detection for diagnostic purposes from clinical samples in the setting of a basic research molecular biology lab. The procedure details the necessary steps for daily analysis of up to 500 clinical samples with a team composed of 12 experienced researchers. The protocol has been designed to rely on widely available reagents and devices, to cope with heterogeneous clinical specimens, to guarantee nucleic acid extraction from very scarce biological material, and to minimize the rate of false-negative results.

Original language	English
Journal	Methods and Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.3390/mps3030059
Publication status	Published - Sept 2020

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Coupeau, D., Burton, N., Lejeune, N., Loret, S., Petit, A., Pejaković, S., Poulain, F., Bonil, L., Trozzi, G., Wiggers, L., Willemart, K., André, E., Laenen, L., Cuypers, L., Ranst, M. V., Bogaerts, P., Muylkens, B., & gillet, N. (2020). SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab. Methods and Protocols, 3(3). https://doi.org/10.3390/mps3030059

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title = "SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab",

abstract = "The emergence of the SARS-CoV-2 virus and the exponential growth of COVID-19 cases have created a major crisis for public health systems. The critical identification of contagious asymptomatic carriers requires the isolation of viral nucleic acids, reverse transcription, and amplification by PCR. However, the shortage of specific proprietary reagents or the lack of automated platforms have seriously hampered diagnostic throughput in many countries. Here, we provide a procedure for SARS-CoV-2 detection for diagnostic purposes from clinical samples in the setting of a basic research molecular biology lab. The procedure details the necessary steps for daily analysis of up to 500 clinical samples with a team composed of 12 experienced researchers. The protocol has been designed to rely on widely available reagents and devices, to cope with heterogeneous clinical specimens, to guarantee nucleic acid extraction from very scarce biological material, and to minimize the rate of false-negative results.",

author = "Damien Coupeau and Nicolas Burton and No{\'e}mie Lejeune and Suzanne Loret and Astrid Petit and Sr{\d}an Pejakovi{\'c} and Florian Poulain and Laura Bonil and Gabrielle Trozzi and Laetitia Wiggers and K{\'e}vin Willemart and Emmanuel Andr{\'e} and Lies Laenen and Lize Cuypers and Ranst, {Marc Van} and Pierre Bogaerts and Beno{\^i}t Muylkens and nicolas gillet",

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year = "2020",

month = sep,

doi = "10.3390/mps3030059",

language = "English",

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Coupeau, D , Burton, N , Lejeune, N, Loret, S, Petit, A , Pejaković, S , Poulain, F , Bonil, L, Trozzi, G, Wiggers, L , Willemart, K, André, E, Laenen, L, Cuypers, L, Ranst, MV, Bogaerts, P, Muylkens, B & gillet, N 2020, 'SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab', Methods and Protocols, vol. 3, no. 3. https://doi.org/10.3390/mps3030059

TY - JOUR

T1 - SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab

AU - Coupeau, Damien

AU - Burton, Nicolas

AU - Lejeune, Noémie

AU - Loret, Suzanne

AU - Petit, Astrid

AU - Pejaković, Srđan

AU - Poulain, Florian

AU - Bonil, Laura

AU - Trozzi, Gabrielle

AU - Wiggers, Laetitia

AU - Willemart, Kévin

AU - André, Emmanuel

AU - Laenen, Lies

AU - Cuypers, Lize

AU - Ranst, Marc Van

AU - Bogaerts, Pierre

AU - Muylkens, Benoît

AU - gillet, nicolas

PY - 2020/9

Y1 - 2020/9

N2 - The emergence of the SARS-CoV-2 virus and the exponential growth of COVID-19 cases have created a major crisis for public health systems. The critical identification of contagious asymptomatic carriers requires the isolation of viral nucleic acids, reverse transcription, and amplification by PCR. However, the shortage of specific proprietary reagents or the lack of automated platforms have seriously hampered diagnostic throughput in many countries. Here, we provide a procedure for SARS-CoV-2 detection for diagnostic purposes from clinical samples in the setting of a basic research molecular biology lab. The procedure details the necessary steps for daily analysis of up to 500 clinical samples with a team composed of 12 experienced researchers. The protocol has been designed to rely on widely available reagents and devices, to cope with heterogeneous clinical specimens, to guarantee nucleic acid extraction from very scarce biological material, and to minimize the rate of false-negative results.

AB - The emergence of the SARS-CoV-2 virus and the exponential growth of COVID-19 cases have created a major crisis for public health systems. The critical identification of contagious asymptomatic carriers requires the isolation of viral nucleic acids, reverse transcription, and amplification by PCR. However, the shortage of specific proprietary reagents or the lack of automated platforms have seriously hampered diagnostic throughput in many countries. Here, we provide a procedure for SARS-CoV-2 detection for diagnostic purposes from clinical samples in the setting of a basic research molecular biology lab. The procedure details the necessary steps for daily analysis of up to 500 clinical samples with a team composed of 12 experienced researchers. The protocol has been designed to rely on widely available reagents and devices, to cope with heterogeneous clinical specimens, to guarantee nucleic acid extraction from very scarce biological material, and to minimize the rate of false-negative results.

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DO - 10.3390/mps3030059

M3 - Article

C2 - 32824827

SN - 2409-9279

VL - 3

JO - Methods and Protocols

JF - Methods and Protocols

IS - 3

ER -

SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab

Abstract

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