Role of PECAM-1 in the adherence of PMN to hypoxic endothelial cells

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Abstract

Hypoxia induces an increase in PMN adherence to endothelial cells for which an interaction between ICAM-1 and CD18/CD11b has been demonstrated. Since PECAM-1 has been shown to be involved in PMN transmigration through the endothelium and to increase the binding capacity of leukocyte CD18/CD11b, the role of this molecule in the hypoxia-induced PMN adherence was investigated. Hypoxia did not change the total surface expression of PECAM-1 on HUVEC and did not change the cell-cell border localization of this molecule as TNF-α did. In addition, blocking anti-PECAM-1 antibodies could not inhibit the increased adherence of unstimulated human PMN to hypoxia-incubated HUVEC while anti-ICAM-1 partially inhibited this process. These results indicate that PECAM-1 is probably not involved in the hypoxia-induced PMN adherence to endothelial cells.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalCell adhesion and communication
Volume5
Issue number5
Publication statusPublished - 1998

Keywords

  • Endothelial cells
  • Hypoxia
  • Neutrophils
  • PECAM-1

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