Research output per year
Research output per year
Émilie Faway, Ludivine Cambier, Evelyne De Vuyst, Céline Evrard, Marc Thiry, Catherine Lambert De Rouvroit, Bernard Mignon, Yves Poumay
Research output: Contribution to journal › Article › peer-review
Despite the threatening incidence of dermatophytosis, information is still lacking about the consequences of infection on epidermal barrier functions and about the keratinocyte responses that alert immune components. To identify the mechanisms involved, arthroconidia of the anthropophilic dermatophyte Trichophyton rubrum were prepared to infect reconstructed human epidermis (RHE) in vitro. Integrity of the barrier was monitored during infection by measurements of transepithelial electrical resistance and dye-permeation through the RHE. Expression and release of pro-inflammatory cytokines and antimicrobial peptides by keratinocytes inserted into the RHE were assessed, respectively, by quantitative reverse transcriptase–PCR (to analyze mRNA content in tissue extracts) and by ELISA (to detect proteins in culture media). Results reveal that infection by T. rubrum is responsible for disruption of the epidermal barrier, including loss of functional tight junctions. It additionally causes simultaneous expression and release of cytokines and antimicrobial peptides by keratinocytes. Potential involvement of the p38 mitogen-activated protein kinase signaling pathway was evaluated during infection by targeted inhibition of its activity. Intriguingly, among several p38 mitogen-activated protein kinase inhibitors, PD169316 alone was able to inhibit growth of T. rubrum on Sabouraud agar and to suppress the process of infection on RHE. This suggests that PD169316 acts on a specific target in dermatophytes themselves.
Original language | English |
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Pages (from-to) | 2080-2089.e6 |
Number of pages | 50 |
Journal | The journal of investigative dermatology |
Volume | 139 |
Issue number | 10 |
Early online date | 15 Apr 2019 |
DOIs | |
Publication status | Published - 1 Oct 2019 |
Research output: Contribution to journal › Review article › peer-review
Research output: Contribution to conference › Abstract › peer-review
29/01/14 → 29/01/17
Project: Research
Poumay, Y. (Creator) & Faway, E. (Data Manager), University of Namur, 28 Feb 2019
Dataset
Supervisor: Lambert De Rouvroit, C. (Co-Supervisor), Poumay, Y. (Supervisor), De Bolle, X. (President), Del-Marmol, V. (External person) (Jury), Jonca, N. (External person) (Jury), Mignon, B. R. (External person) (Jury) & Monod, M. (External person) (Jury)
Student thesis: Doc types › Doctor of Biomedical and Pharmaceutical Sciences