Repression of Cell Differentiation by a cis-Acting lincRNA in Fission Yeast

Research output: Contribution to journalArticle

Abstract

The cell fate decision leading to gametogenesis requires the convergence of multiple signals on the promoter of a master regulator. In fission yeast, starvation-induced signaling leads to the transcriptional induction of the ste11 gene, which encodes the central inducer of mating and gametogenesis, known as sporulation. We find that the long intergenic non-coding (linc) RNA rse1 is transcribed divergently upstream of the ste11 gene. During vegetative growth, rse1 directly recruits a Mug187-Lid2-Set1 complex that mediates cis repression at the ste11 promoter through SET3C-dependent histone deacetylation. The absence of rse1 bypasses the starvation-induced signaling and induces gametogenesis in the presence of nutrients. Our data reveal that the remodeling of chromatin through ncRNA scaffolding of repressive complexes that is observed in higher eukaryotes is a conserved, likely very ancient mechanism for tight control of cell differentiation. Fauquenoy et al. report the first example of a yeast long intergenic non-coding RNA (lincRNA) that regulates the expression of the neighboring ste11 gene through scaffolding of a repressive complex. This constitutes an RNA-based mechanism of repression of cell differentiation.

Original languageEnglish
Pages (from-to)383-391.e3
JournalCurrent biology : CB
Volume28
Issue number3
Early online date2018
DOIs
Publication statusPublished - 5 Feb 2018

Fingerprint

Long Noncoding RNA
Gametogenesis
Untranslated RNA
Schizosaccharomyces
Schizosaccharomyces pombe
gametogenesis
cell differentiation
Yeast
Cell Differentiation
Genes
Starvation
starvation
promoter regions
Chromatin Assembly and Disassembly
gene induction
Eukaryota
sporulation
histones
Histones
Nutrients

Keywords

  • differentiation
  • ncRNA
  • transcription
  • yeast

Cite this

@article{64a9ca88336e4dc4b14eb5a47cdd6439,
title = "Repression of Cell Differentiation by a cis-Acting lincRNA in Fission Yeast",
abstract = "The cell fate decision leading to gametogenesis requires the convergence of multiple signals on the promoter of a master regulator. In fission yeast, starvation-induced signaling leads to the transcriptional induction of the ste11 gene, which encodes the central inducer of mating and gametogenesis, known as sporulation. We find that the long intergenic non-coding (linc) RNA rse1 is transcribed divergently upstream of the ste11 gene. During vegetative growth, rse1 directly recruits a Mug187-Lid2-Set1 complex that mediates cis repression at the ste11 promoter through SET3C-dependent histone deacetylation. The absence of rse1 bypasses the starvation-induced signaling and induces gametogenesis in the presence of nutrients. Our data reveal that the remodeling of chromatin through ncRNA scaffolding of repressive complexes that is observed in higher eukaryotes is a conserved, likely very ancient mechanism for tight control of cell differentiation. Fauquenoy et al. report the first example of a yeast long intergenic non-coding RNA (lincRNA) that regulates the expression of the neighboring ste11 gene through scaffolding of a repressive complex. This constitutes an RNA-based mechanism of repression of cell differentiation.",
keywords = "differentiation, ncRNA, transcription, yeast",
author = "Sylvain Fauquenoy and Valerie Migeot and Olivier Finet and Carlo Yague-Sanz and Olga Khorosjutina and Karl Ekwall and Damien Hermand",
note = "Copyright {\circledC} 2017 Elsevier Ltd. All rights reserved.",
year = "2018",
month = "2",
day = "5",
doi = "https://doi.org/10.1016/j.cub.2017.12.048",
language = "English",
volume = "28",
pages = "383--391.e3",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "3",

}

Repression of Cell Differentiation by a cis-Acting lincRNA in Fission Yeast. / Fauquenoy, Sylvain; Migeot, Valerie; Finet, Olivier; Yague-Sanz, Carlo; Khorosjutina, Olga; Ekwall, Karl; Hermand, Damien.

In: Current biology : CB, Vol. 28, No. 3, 05.02.2018, p. 383-391.e3.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Repression of Cell Differentiation by a cis-Acting lincRNA in Fission Yeast

AU - Fauquenoy, Sylvain

AU - Migeot, Valerie

AU - Finet, Olivier

AU - Yague-Sanz, Carlo

AU - Khorosjutina, Olga

AU - Ekwall, Karl

AU - Hermand, Damien

N1 - Copyright © 2017 Elsevier Ltd. All rights reserved.

PY - 2018/2/5

Y1 - 2018/2/5

N2 - The cell fate decision leading to gametogenesis requires the convergence of multiple signals on the promoter of a master regulator. In fission yeast, starvation-induced signaling leads to the transcriptional induction of the ste11 gene, which encodes the central inducer of mating and gametogenesis, known as sporulation. We find that the long intergenic non-coding (linc) RNA rse1 is transcribed divergently upstream of the ste11 gene. During vegetative growth, rse1 directly recruits a Mug187-Lid2-Set1 complex that mediates cis repression at the ste11 promoter through SET3C-dependent histone deacetylation. The absence of rse1 bypasses the starvation-induced signaling and induces gametogenesis in the presence of nutrients. Our data reveal that the remodeling of chromatin through ncRNA scaffolding of repressive complexes that is observed in higher eukaryotes is a conserved, likely very ancient mechanism for tight control of cell differentiation. Fauquenoy et al. report the first example of a yeast long intergenic non-coding RNA (lincRNA) that regulates the expression of the neighboring ste11 gene through scaffolding of a repressive complex. This constitutes an RNA-based mechanism of repression of cell differentiation.

AB - The cell fate decision leading to gametogenesis requires the convergence of multiple signals on the promoter of a master regulator. In fission yeast, starvation-induced signaling leads to the transcriptional induction of the ste11 gene, which encodes the central inducer of mating and gametogenesis, known as sporulation. We find that the long intergenic non-coding (linc) RNA rse1 is transcribed divergently upstream of the ste11 gene. During vegetative growth, rse1 directly recruits a Mug187-Lid2-Set1 complex that mediates cis repression at the ste11 promoter through SET3C-dependent histone deacetylation. The absence of rse1 bypasses the starvation-induced signaling and induces gametogenesis in the presence of nutrients. Our data reveal that the remodeling of chromatin through ncRNA scaffolding of repressive complexes that is observed in higher eukaryotes is a conserved, likely very ancient mechanism for tight control of cell differentiation. Fauquenoy et al. report the first example of a yeast long intergenic non-coding RNA (lincRNA) that regulates the expression of the neighboring ste11 gene through scaffolding of a repressive complex. This constitutes an RNA-based mechanism of repression of cell differentiation.

KW - differentiation

KW - ncRNA

KW - transcription

KW - yeast

UR - http://www.scopus.com/inward/record.url?scp=85041630378&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.cub.2017.12.048

DO - https://doi.org/10.1016/j.cub.2017.12.048

M3 - Article

C2 - 29395921

VL - 28

SP - 383-391.e3

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 3

ER -