Abstract
This work shows that the proximal promoter of the mouse Afp gene contains a Ku binding site and that Ku binding is associated with down-regulation of the transcriptional activity of the Afp promoter. The Ku binding site is located in a segment able to adopt a peculiar structured form, probably a hairpin structure. Interestingly, the structured form eliminates the binding sites of the positive transcription factor HNF1. Furthermore, a DNAse hypersensitive site is detected in footprinting experiments done with extracts of AFP non-expressing hepatoma cells. These observations suggest that the structured form is stabilised by Ku and is associated with extinction of the gene in AFP non-expressing hepatic cells. © 2006 Elsevier Masson SAS. All rights reserved.
Original language | English |
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Pages (from-to) | 1409-1417 |
Number of pages | 9 |
Journal | Biochimie |
Volume | 88 |
Issue number | 10 |
DOIs | |
Publication status | Published - Oct 2006 |
Keywords
- Alpha-fetoprotein
- DNA conformation
- Ku
- Transcription
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