Reconstruction of normal and pathological human epidermis on polycarbonate filter

Evelyne De Vuyst; Céline Charlier; Séverine Giltaire; Valérie De Glas; Catherine Lambert De Rouvroit; Yves Poumay

doi:10.1007/7651-2013-40

Reconstruction of normal and pathological human epidermis on polycarbonate filter

Evelyne De Vuyst, Céline Charlier, Séverine Giltaire, Valérie De Glas, Catherine Lambert De Rouvroit, Yves Poumay

Research output: Contribution in Book/Catalog/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

147 Downloads (Pure)

Abstract

This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.

Original language	English
Title of host publication	Epidermal cells 3rd Edition
Subtitle of host publication	Methods in Molecular Biology
Editors	Kursad Turksen
Place of Publication	Clifton, N.J.
Publisher	Humana Press
Pages	191-201
Number of pages	11
Volume	1195
ISBN (Print)	9781493912230
DOIs	https://doi.org/10.1007/7651-2013-40
Publication status	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1195
ISSN (Print)	10643745

Keywords

Darier's disease
Epidermis reconstruction
Human keratinocytes
Primary culture
Skin biopsy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/7651-2013-40

Epidermal Cell-Chapter De Vuyst et alSubmitted manuscript, 457 KB

Cite this

De Vuyst, E., Charlier, C., Giltaire, S., De Glas, V., De Rouvroit, C. L., & Poumay, Y. (2014). Reconstruction of normal and pathological human epidermis on polycarbonate filter. In K. Turksen (Ed.), Epidermal cells 3rd Edition: Methods in Molecular Biology (Vol. 1195, pp. 191-201). (Methods in Molecular Biology; Vol. 1195). Humana Press. https://doi.org/10.1007/7651-2013-40

@inbook{bef47a2ac62b44769ca48f67c12d2768,

title = "Reconstruction of normal and pathological human epidermis on polycarbonate filter",

abstract = "This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.",

keywords = "Darier's disease, Epidermis reconstruction, Human keratinocytes, Primary culture, Skin biopsy",

author = "{De Vuyst}, Evelyne and C{\'e}line Charlier and S{\'e}verine Giltaire and {De Glas}, Val{\'e}rie and {De Rouvroit}, {Catherine Lambert} and Yves Poumay",

note = "ISSN 1064-3745",

year = "2014",

doi = "10.1007/7651-2013-40",

language = "English",

isbn = "9781493912230",

volume = "1195",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "191--201",

editor = "Kursad Turksen",

booktitle = "Epidermal cells 3rd Edition",

}

De Vuyst, E, Charlier, C, Giltaire, S, De Glas, V, De Rouvroit, CL & Poumay, Y 2014, Reconstruction of normal and pathological human epidermis on polycarbonate filter. in K Turksen (ed.), Epidermal cells 3rd Edition: Methods in Molecular Biology. vol. 1195, Methods in Molecular Biology, vol. 1195, Humana Press, Clifton, N.J., pp. 191-201. https://doi.org/10.1007/7651-2013-40

Reconstruction of normal and pathological human epidermis on polycarbonate filter. / De Vuyst, Evelyne; Charlier, Céline; Giltaire, Séverine et al.
Epidermal cells 3rd Edition: Methods in Molecular Biology. ed. / Kursad Turksen. Vol. 1195 Clifton, N.J.: Humana Press, 2014. p. 191-201 (Methods in Molecular Biology; Vol. 1195).

Research output: Contribution in Book/Catalog/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Reconstruction of normal and pathological human epidermis on polycarbonate filter

AU - De Vuyst, Evelyne

AU - Charlier, Céline

AU - Giltaire, Séverine

AU - De Glas, Valérie

AU - De Rouvroit, Catherine Lambert

AU - Poumay, Yves

N1 - ISSN 1064-3745

PY - 2014

Y1 - 2014

N2 - This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.

AB - This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.

KW - Darier's disease

KW - Epidermis reconstruction

KW - Human keratinocytes

KW - Primary culture

KW - Skin biopsy

UR - http://www.scopus.com/inward/record.url?scp=84907338599&partnerID=8YFLogxK

U2 - 10.1007/7651-2013-40

DO - 10.1007/7651-2013-40

M3 - Chapter (peer-reviewed)

C2 - 24155233

AN - SCOPUS:84907338599

SN - 9781493912230

VL - 1195

T3 - Methods in Molecular Biology

SP - 191

EP - 201

BT - Epidermal cells 3rd Edition

A2 - Turksen, Kursad

PB - Humana Press

CY - Clifton, N.J.

ER -

Reconstruction of normal and pathological human epidermis on polycarbonate filter

Abstract

Publication series

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Study of the disruption of the barrier of reconstructed epidermis in vitro in order to mimic the epidermal characteristics of the atopic dermatitis

Histology platform

Barrier alterations in a reconstructed epidermis as a model for atopic dermatitis

Cite this

Reconstruction of normal and pathological human epidermis on polycarbonate filter

Abstract

Publication series

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

Study of the disruption of the barrier of reconstructed epidermis in vitro in order to mimic the epidermal characteristics of the atopic dermatitis

Equipment

Histology platform

Student theses

Barrier alterations in a reconstructed epidermis as a model for atopic dermatitis

Cite this