Reconstruction of normal and pathological human epidermis on polycarbonate filter

Evelyne De Vuyst, Céline Charlier, Séverine Giltaire, Valérie De Glas, Catherine Lambert De Rouvroit, Yves Poumay

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Abstract

This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.

Original languageEnglish
Title of host publicationEpidermal cells 3rd Edition
Subtitle of host publicationMethods in Molecular Biology
EditorsKursad Turksen
Place of PublicationClifton, N.J.
PublisherHumana Press
Pages191-201
Number of pages11
Volume1195
ISBN (Print)9781493912230
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1195
ISSN (Print)10643745

Keywords

  • Darier's disease
  • Epidermis reconstruction
  • Human keratinocytes
  • Primary culture
  • Skin biopsy

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  • Projects

    Equipment

  • Student Theses

    Barrier alterations in a reconstructed epidermis as a model for atopic dermatitis

    Author: De Vuyst, E., 20 Dec 2016

    Supervisor: Poumay, Y. (Supervisor), Lambert De Rouvroit, C. (Co-Supervisor), Michiels, C. (President), Gillet, J. (Jury), Brandner, J. (External person) (Jury), Salmon, M. (External person) (Jury) & Nikkels, A. F. (External person) (Jury)

    Student thesis: Doc typesDoctor of Biomedical and Pharmaceutical Sciences

    Cite this

    De Vuyst, E., Charlier, C., Giltaire, S., De Glas, V., De Rouvroit, C. L., & Poumay, Y. (2014). Reconstruction of normal and pathological human epidermis on polycarbonate filter. In K. Turksen (Ed.), Epidermal cells 3rd Edition: Methods in Molecular Biology (Vol. 1195, pp. 191-201). (Methods in Molecular Biology; Vol. 1195). Humana Press. https://doi.org/10.1007/7651-2013-40