Projects per year
Abstract
This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Darier's disease and the reconstruction of a pathological epidermis which displays the same histological features as in vivo are also presented.
Original language | English |
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Title of host publication | Epidermal cells 3rd Edition |
Subtitle of host publication | Methods in Molecular Biology |
Editors | Kursad Turksen |
Place of Publication | Clifton, N.J. |
Publisher | Humana Press |
Pages | 191-201 |
Number of pages | 11 |
Volume | 1195 |
ISBN (Print) | 9781493912230 |
DOIs | |
Publication status | Published - 2014 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1195 |
ISSN (Print) | 10643745 |
Keywords
- Darier's disease
- Epidermis reconstruction
- Human keratinocytes
- Primary culture
- Skin biopsy
Fingerprint
Dive into the research topics of 'Reconstruction of normal and pathological human epidermis on polycarbonate filter'. Together they form a unique fingerprint.Projects
- 1 Finished
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Study of the disruption of the barrier of reconstructed epidermis in vitro in order to mimic the epidermal characteristics of the atopic dermatitis
DE VUYST, E. (Researcher) & Poumay, Y. (CoI)
14/03/12 → 20/12/16
Project: PHD
Equipment
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Histology platform
Nicaise, C. (Manager), Poumay, Y. (Manager) & Bielarz, V. (Other)
Technological Platform Morphology - ImagingFacility/equipment: Equipment
Student theses
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Barrier alterations in a reconstructed epidermis as a model for atopic dermatitis
De Vuyst, E. (Author)Poumay, Y. (Supervisor), Lambert De Rouvroit, C. (Co-Supervisor), Michiels, C. (President), Gillet, J.-P. (Jury), Brandner, J. (Jury), Salmon, M. (Jury) & Nikkels, A. F. (Jury), 20 Dec 2016Student thesis: Doc types › Doctor of Biomedical and Pharmaceutical Sciences