Reciprocal regulation of TORC signaling and tRNA modifications by Elongator enforces nutrient-dependent cell fate

Julie Candiracci, Valerie Migeot, Yok-Hian Chionh, Fanelie Bauer, Thomas Brochier, Brandon Russell, Kazuhiro Shiozaki, Peter Dedon, Damien Hermand

Research output: Contribution to journalArticle

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Abstract

Nutrient availability has a profound impact on cell fate. Upon nitrogen starvation, wild-type fission yeast cells uncouple cell growth from cell division to generate small, round-shaped cells that are competent for sexual differentiation. The TORC1 (TOR complex 1) and TORC2 complexes exert opposite controls on cell growth and cell differentiation, but little is known about how their activity is coordinated. We show that transfer RNA (tRNA) modifications by Elongator are critical for this regulation by promoting the translation of both key components of TORC2 and repressors of TORC1. We further identified the TORC2 pathway as an activator of Elongator by down-regulating a Gsk3 (glycogen synthase kinase 3)-dependent inhibitory phosphorylation of Elongator. Therefore, a feedback control is operating between TOR complex (TORC) signaling and tRNA modification by Elongator to enforce the advancement of mitosis that precedes cell differentiation.

Original languageEnglish
Article numbereaav0184
Pages (from-to)eaav0184
Number of pages13
JournalScience Advances
Volume5
Issue number6
DOIs
Publication statusPublished - 1 Jan 2019

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Transfer RNA
Food
Cell Differentiation
Glycogen Synthase Kinase 3
Sex Differentiation
Schizosaccharomyces
Growth
Starvation
Mitosis
Cell Division
Nitrogen
Phosphorylation
TOR complex 2
mechanistic target of rapamycin complex 1

Cite this

Candiracci, Julie ; Migeot, Valerie ; Chionh, Yok-Hian ; Bauer, Fanelie ; Brochier, Thomas ; Russell, Brandon ; Shiozaki, Kazuhiro ; Dedon, Peter ; Hermand, Damien. / Reciprocal regulation of TORC signaling and tRNA modifications by Elongator enforces nutrient-dependent cell fate. In: Science Advances. 2019 ; Vol. 5, No. 6. pp. eaav0184.
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Reciprocal regulation of TORC signaling and tRNA modifications by Elongator enforces nutrient-dependent cell fate. / Candiracci, Julie; Migeot, Valerie; Chionh, Yok-Hian; Bauer, Fanelie; Brochier, Thomas; Russell, Brandon; Shiozaki, Kazuhiro; Dedon, Peter; Hermand, Damien.

In: Science Advances, Vol. 5, No. 6, eaav0184, 01.01.2019, p. eaav0184.

Research output: Contribution to journalArticle

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AU - Candiracci, Julie

AU - Migeot, Valerie

AU - Chionh, Yok-Hian

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AU - Brochier, Thomas

AU - Russell, Brandon

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AU - Dedon, Peter

AU - Hermand, Damien

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