Rapid exclusion of the diagnosis of immune HIT by AcuStar HIT and heparin-induced multiple electrode aggregometry

Research output: Contribution to journalArticle

266 Downloads (Pure)

Abstract

Background Accurate diagnosis of heparin-induced thrombocytopenia (HIT) is essential but remains challenging. We have previously demonstrated, in a retrospective study, the usefulness of the combination of the 4Ts score, AcuStar HIT and heparin-induced multiple electrode aggregometry (HIMEA) with optimized thresholds. Objectives We aimed at exploring prospectively the performances of our optimized diagnostic algorithm on suspected HIT patients. The secondary objective is to evaluate performances of AcuStar HIT-Ab (PF4-H) in comparison with the clinical outcome. Methods 116 inpatients with clinically suspected immune HIT were included. Our optimized diagnostic algorithm was applied to each patient. Sensitivity, specificity, negative predictive value (NPV), positive predictive value (PPV) of the overall diagnostic strategy as well as AcuStar HIT-Ab (at manufacturer's thresholds and at our thresholds) were calculated using clinical diagnosis as the reference. Results Among 116 patients, 2 patients had clinically-diagnosed HIT. These 2 patients were positive on AcuStar HIT-Ab, AcuStar HIT-IgG and HIMEA. Using our optimized algorithm, all patients were correctly diagnosed. AcuStar HIT-Ab at our cut-off (> 9.41 U/mL) and at manufacturer's cut-off (> 1.00 U/mL) showed both a sensitivity of 100.0% and a specificity of 99.1% and 90.4%, respectively. Conclusion The combination of the 4Ts score, the HemosIL® AcuStar HIT and HIMEA with optimized thresholds may be useful for the rapid and accurate exclusion of the diagnosis of immune HIT. © 2014 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1074-1078
Number of pages5
JournalThrombosis Research
Volume133
Issue number6
DOIs
Publication statusPublished - 30 Jun 2014

Keywords

  • HemosIL AcuStar HIT
  • heparin-induced multiple electrode aggregometry
  • immune heparin-induced thrombocytopenia

Fingerprint Dive into the research topics of 'Rapid exclusion of the diagnosis of immune HIT by AcuStar HIT and heparin-induced multiple electrode aggregometry'. Together they form a unique fingerprint.

  • Student Theses

    Analytical and clinical validation of sensitive assays for the diagnosis of immune heparin-induced thrombocytopenia

    Author: Minet, V., 2017

    Supervisor: Dogne, J. (Supervisor), Mullier, F. (External person) (Co-Supervisor), Laloy, J. (Jury), Lucas, S. (Jury), Masereel, B. (Jury), Chatelain, M. (External person) (Jury), Hoylaerts, M. F. (External person) (Jury) & Tardy, B. (External person) (Jury)

    Student thesis: Doc typesDoctor of Biomedical and Pharmaceutical Sciences

    Cite this