Quality control data of physiological and immunological biomarkers measured in serum and plasma

Eugène Jansen; Piet Beekhof; Johannes Cremers; Birgit Weinberger; Simone Fiegl; Olivier Toussaint; Jürgen Bernhard; Efstathios Gonos; Miriam Capri; Claudio Franceschi; Ewa Sikora; María Moreno-Villanueva; Nicolle Breusing; Tilman Grune; Alexander Bürkle; Martijn E T Dollé

doi:10.1016/j.mad.2015.06.004

Quality control data of physiological and immunological biomarkers measured in serum and plasma

Eugène Jansen, Piet Beekhof, Johannes Cremers, Birgit Weinberger, Simone Fiegl, Olivier Toussaint, Jürgen Bernhard, Efstathios Gonos, Miriam Capri, Claudio Franceschi, Ewa Sikora, María Moreno-Villanueva, Nicolle Breusing, Tilman Grune, Alexander Bürkle, Martijn E T Dollé

Laboratory of Cellular Biochemistry and Biology

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Abstract

In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years.The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples.Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values.A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up.Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.

Original language	English
Pages (from-to)	54-59
Number of pages	6
Journal	Mechanisms of Ageing and Development
Volume	151
DOIs	https://doi.org/10.1016/j.mad.2015.06.004
Publication status	Published - 30 Jan 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 1 No Poverty

Keywords

Biomarkers
Hemolysis
Quality control

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10.1016/j.mad.2015.06.004Licence: CC BY-NC-ND

1_s2.0_S0047637415300014_mainFinal published version, 625 KBLicence: CC BY-NC-ND

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Jansen, E., Beekhof, P., Cremers, J., Weinberger, B., Fiegl, S., Toussaint, O., Bernhard, J., Gonos, E., Capri, M., Franceschi, C., Sikora, E., Moreno-Villanueva, M., Breusing, N., Grune, T., Bürkle, A., & Dollé, M. E. T. (2015). Quality control data of physiological and immunological biomarkers measured in serum and plasma. Mechanisms of Ageing and Development, 151, 54-59. https://doi.org/10.1016/j.mad.2015.06.004

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title = "Quality control data of physiological and immunological biomarkers measured in serum and plasma",

abstract = "In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years.The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2\%) that were rejected because of a high hemolysis, icteria or lipemia of the samples.Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8\%, with an average inter-assay coefficient of variation of all biomarkers of 4.0\%. Also the precision of the measurements was very good, because all measurements were between 90\% and 115\% of the defined target values.A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3\% of the sample identification could be mixed-up.Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.",

keywords = "Biomarkers, Hemolysis, Quality control",

author = "Eug{\`e}ne Jansen and Piet Beekhof and Johannes Cremers and Birgit Weinberger and Simone Fiegl and Olivier Toussaint and J{\"u}rgen Bernhard and Efstathios Gonos and Miriam Capri and Claudio Franceschi and Ewa Sikora and Mar{\'i}a Moreno-Villanueva and Nicolle Breusing and Tilman Grune and Alexander B{\"u}rkle and Doll{\'e}, \{Martijn E T\}",

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year = "2015",

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day = "30",

doi = "10.1016/j.mad.2015.06.004",

language = "English",

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Jansen, E, Beekhof, P, Cremers, J, Weinberger, B, Fiegl, S, Toussaint, O, Bernhard, J, Gonos, E, Capri, M, Franceschi, C, Sikora, E, Moreno-Villanueva, M, Breusing, N, Grune, T, Bürkle, A & Dollé, MET 2015, 'Quality control data of physiological and immunological biomarkers measured in serum and plasma', Mechanisms of Ageing and Development, vol. 151, pp. 54-59. https://doi.org/10.1016/j.mad.2015.06.004

TY - JOUR

T1 - Quality control data of physiological and immunological biomarkers measured in serum and plasma

AU - Jansen, Eugène

AU - Beekhof, Piet

AU - Cremers, Johannes

AU - Weinberger, Birgit

AU - Fiegl, Simone

AU - Toussaint, Olivier

AU - Bernhard, Jürgen

AU - Gonos, Efstathios

AU - Capri, Miriam

AU - Franceschi, Claudio

AU - Sikora, Ewa

AU - Moreno-Villanueva, María

AU - Breusing, Nicolle

AU - Grune, Tilman

AU - Bürkle, Alexander

AU - Dollé, Martijn E T

PY - 2015/1/30

Y1 - 2015/1/30

N2 - In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years.The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples.Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values.A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up.Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.

AB - In two work packages of the MARK-AGE project, 37 immunological and physiological biomarkers were measured in 3637 serum, plasma or blood samples in five batches during a period of 4 years.The quality of the serum and plasma samples was very good as judged by the low number of biomarker measurements (only 0.2%) that were rejected because of a high hemolysis, icteria or lipemia of the samples.Using quality control samples, day-to-day and batch variations were determined. The mean inter-assay variation of the five batches were all below 8%, with an average inter-assay coefficient of variation of all biomarkers of 4.0%. Also the precision of the measurements was very good, because all measurements were between 90% and 115% of the defined target values.A possible mix-up of samples was determined by comparison of the extreme testosterone levels of men and women. It was concluded that 3% of the sample identification could be mixed-up.Considering the complex procedure from collection to analysis, including preparation, handling, shipment and storage, of the samples in the MARK-AGE project, both the quality of the samples and the quality of the measurements are very good.

KW - Biomarkers

KW - Hemolysis

KW - Quality control

UR - https://www.scopus.com/pages/publications/84947040058

U2 - 10.1016/j.mad.2015.06.004

DO - 10.1016/j.mad.2015.06.004

M3 - Article

C2 - 26166476

SN - 0047-6374

VL - 151

SP - 54

EP - 59

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

ER -

Quality control data of physiological and immunological biomarkers measured in serum and plasma

Abstract

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Keywords

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