Abstract
To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA-like protein, an oxido-reductase, a heat-shock protein and an ATP synthase β mitochondrial precursor. The possible fungal origin of a MYK15-like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.
Original language | English |
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Pages (from-to) | 401-411 |
Number of pages | 11 |
Journal | FEMS Microbiology Ecology |
Volume | 48 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Jun 2004 |
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Mass Spectrometry Service
Renard, P. (Manager)
Technological Platform Mass Spectrometry ServiceFacility/equipment: Technological Platform