Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas

E. Dumas-Gaudot, B. Valot, G. Bestel-Corre, G. Recorbet, M. St-Arnaud, B. Fontaine, M. Dieu, Martine Raes, R.S. Saravanan, S. Gianinazzi

Research output: Contribution to journalArticle

Abstract

To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA-like protein, an oxido-reductase, a heat-shock protein and an ATP synthase β mitochondrial precursor. The possible fungal origin of a MYK15-like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.
Original languageEnglish
Pages (from-to)401-411
Number of pages11
JournalFEMS Microbiology Ecology
Volume48
Issue number3
DOIs
Publication statusPublished - 1 Jun 2004

Fingerprint

Daucus carota
Fungal Proteins
proteomics
Proteomics
DNA
protein
Proteins
Mitochondrial Proton-Translocating ATPases
Symbiosis
Electrophoresis, Gel, Two-Dimensional
Heat-Shock Proteins
Tandem Mass Spectrometry
Oxidoreductases
Fungi
heat shock
symbiosis
electrokinesis
gel
mass spectrometry
fungus

Cite this

Dumas-Gaudot, E., Valot, B., Bestel-Corre, G., Recorbet, G., St-Arnaud, M., Fontaine, B., ... Gianinazzi, S. (2004). Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas. FEMS Microbiology Ecology, 48(3), 401-411. https://doi.org/10.1016/j.femsec.2004.02.015
Dumas-Gaudot, E. ; Valot, B. ; Bestel-Corre, G. ; Recorbet, G. ; St-Arnaud, M. ; Fontaine, B. ; Dieu, M. ; Raes, Martine ; Saravanan, R.S. ; Gianinazzi, S. / Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas. In: FEMS Microbiology Ecology. 2004 ; Vol. 48, No. 3. pp. 401-411.
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Dumas-Gaudot, E, Valot, B, Bestel-Corre, G, Recorbet, G, St-Arnaud, M, Fontaine, B, Dieu, M, Raes, M, Saravanan, RS & Gianinazzi, S 2004, 'Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas', FEMS Microbiology Ecology, vol. 48, no. 3, pp. 401-411. https://doi.org/10.1016/j.femsec.2004.02.015

Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas. / Dumas-Gaudot, E.; Valot, B.; Bestel-Corre, G.; Recorbet, G.; St-Arnaud, M.; Fontaine, B.; Dieu, M.; Raes, Martine; Saravanan, R.S.; Gianinazzi, S.

In: FEMS Microbiology Ecology, Vol. 48, No. 3, 01.06.2004, p. 401-411.

Research output: Contribution to journalArticle

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