Proteomic analysis of chicory root identifies proteins typically involved in cold acclimation

Hervé Degand, Anne-Marie Faber, Nicolas Dauchot, Dominique Mingeot, Bernard Watillon, Pierre Van Cutsem, Pierre Morsomme, Marc Boutry

Research output: Contribution to journalArticlepeer-review

Abstract

Chicory (Cichorium intybus) roots contain high amounts of inulin, a fructose polymer used as a storage carbohydrate by the plant and as a human dietary and prebiotic compound. We performed 2-D electrophoretic analysis of proteins from root material before the first freezing period. The proteins were digested with trypsin and the peptides analyzed by MS (MALDI-TOF/TOF). From the 881 protein spots analyzed, 714 proteins corresponded to a database accession, 619 of which were classified into functional categories. Besides expected proteins (e.g. related to metabolism, energy, protein synthesis, or cell structure), other well-represented categories were proteins related to folding and stability (49 spots), proteolysis (49 spots), and the stress response (67 spots). The importance of abiotic stress response was confirmed by the observation that 7 of the 21 most intense protein spots are known to be involved in cold acclimation. These results suggest a major effect of the low temperature period that preceded root harvesting.
Original languageEnglish
Pages (from-to)2903-7
Number of pages5
JournalProteomics
Volume9
Issue number10
DOIs
Publication statusPublished - May 2009

Keywords

  • Plant Roots
  • Trypsin
  • Peptide Mapping
  • Inulin
  • Proteomics
  • Oxidative Stress
  • Electrophoresis, Gel, Two-Dimensional
  • Acclimatization
  • Cold Temperature
  • Chicory
  • Plant Proteins

Fingerprint Dive into the research topics of 'Proteomic analysis of chicory root identifies proteins typically involved in cold acclimation'. Together they form a unique fingerprint.

Cite this