Proteomics technology are increasingly used in ecotoxicological studies to characterize and monitor biomarkers of exposure. The present study aims at identifying long term effects of malachite green (MG) exposure on the proteome of peripheral blood mononuclear cells (PBMC) from the Asian catfish, Pangasianodon hypophthalmus. A common (0.1. ppm) concentration for therapeutic treatment was applied twice with a 72. h interval. PBMC were collected directly at the end of the second bath of MG (T1) and after 1. month of decontamination (T2). Analytical 2D-DIGE gels were run and a total of 2551 ± 364 spots were matched. Among them, MG induced significant changes in abundance of 116 spots with no recovery after one month of decontamination. Using LC-MS/MS and considering single identification per spot, we could identify 25 different proteins. Additionally, MG residues were measured in muscle and in blood indicating that leuco-MG has almost totally disappeared after one month of decontamination. This work highlights long term effects of MG treatment on the PBMC proteome from fish intended for human consumption.
Pierrard, M-A., Kestemont, P., Delaive, E., Thezenas, M-L., Dieu, M., Raes, M., Phuong, N. T., Tran, M. P., & Silvestre, F. (2012). Proteomic analysis of blood cells in fish exposed to chemotherapeutics: Evidence for long term effects. Journal of Proteomics, 75(8), 2454-2467. https://doi.org/10.1016/j.jprot.2012.02.028