Abstract

Proteomics technology are increasingly used in ecotoxicological studies to characterize and monitor biomarkers of exposure. The present study aims at identifying long term effects of malachite green (MG) exposure on the proteome of peripheral blood mononuclear cells (PBMC) from the Asian catfish, Pangasianodon hypophthalmus. A common (0.1. ppm) concentration for therapeutic treatment was applied twice with a 72. h interval. PBMC were collected directly at the end of the second bath of MG (T1) and after 1. month of decontamination (T2). Analytical 2D-DIGE gels were run and a total of 2551 ± 364 spots were matched. Among them, MG induced significant changes in abundance of 116 spots with no recovery after one month of decontamination. Using LC-MS/MS and considering single identification per spot, we could identify 25 different proteins. Additionally, MG residues were measured in muscle and in blood indicating that leuco-MG has almost totally disappeared after one month of decontamination. This work highlights long term effects of MG treatment on the PBMC proteome from fish intended for human consumption.
Original languageEnglish
Pages (from-to)2454-2467
Number of pages14
JournalJournal of Proteomics
Volume75
Issue number8
DOIs
Publication statusPublished - 18 Apr 2012

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Proteomics
Fish
Blood Cells
Fishes
Blood
Cells
Decontamination
Proteome
Two-Dimensional Difference Gel Electrophoresis
Catfishes
Biomarkers
Baths
Muscle
malachite green
Therapeutics
Gels
Technology
Recovery
Muscles
Proteins

Cite this

@article{0278b04fe2404f47909cb6de3d2ed156,
title = "Proteomic analysis of blood cells in fish exposed to chemotherapeutics: Evidence for long term effects",
abstract = "Proteomics technology are increasingly used in ecotoxicological studies to characterize and monitor biomarkers of exposure. The present study aims at identifying long term effects of malachite green (MG) exposure on the proteome of peripheral blood mononuclear cells (PBMC) from the Asian catfish, Pangasianodon hypophthalmus. A common (0.1. ppm) concentration for therapeutic treatment was applied twice with a 72. h interval. PBMC were collected directly at the end of the second bath of MG (T1) and after 1. month of decontamination (T2). Analytical 2D-DIGE gels were run and a total of 2551 ± 364 spots were matched. Among them, MG induced significant changes in abundance of 116 spots with no recovery after one month of decontamination. Using LC-MS/MS and considering single identification per spot, we could identify 25 different proteins. Additionally, MG residues were measured in muscle and in blood indicating that leuco-MG has almost totally disappeared after one month of decontamination. This work highlights long term effects of MG treatment on the PBMC proteome from fish intended for human consumption.",
author = "M.-A. Pierrard and P. Kestemont and E. Delaive and M.-L. Thezenas and M. Dieu and Martine Raes and N.T. Phuong and M.P. Tran and F. Silvestre",
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TY - JOUR

T1 - Proteomic analysis of blood cells in fish exposed to chemotherapeutics

T2 - Evidence for long term effects

AU - Pierrard, M.-A.

AU - Kestemont, P.

AU - Delaive, E.

AU - Thezenas, M.-L.

AU - Dieu, M.

AU - Raes, Martine

AU - Phuong, N.T.

AU - Tran, M.P.

AU - Silvestre, F.

N1 - MEDLINE® is the source for the MeSH terms of this document.

PY - 2012/4/18

Y1 - 2012/4/18

N2 - Proteomics technology are increasingly used in ecotoxicological studies to characterize and monitor biomarkers of exposure. The present study aims at identifying long term effects of malachite green (MG) exposure on the proteome of peripheral blood mononuclear cells (PBMC) from the Asian catfish, Pangasianodon hypophthalmus. A common (0.1. ppm) concentration for therapeutic treatment was applied twice with a 72. h interval. PBMC were collected directly at the end of the second bath of MG (T1) and after 1. month of decontamination (T2). Analytical 2D-DIGE gels were run and a total of 2551 ± 364 spots were matched. Among them, MG induced significant changes in abundance of 116 spots with no recovery after one month of decontamination. Using LC-MS/MS and considering single identification per spot, we could identify 25 different proteins. Additionally, MG residues were measured in muscle and in blood indicating that leuco-MG has almost totally disappeared after one month of decontamination. This work highlights long term effects of MG treatment on the PBMC proteome from fish intended for human consumption.

AB - Proteomics technology are increasingly used in ecotoxicological studies to characterize and monitor biomarkers of exposure. The present study aims at identifying long term effects of malachite green (MG) exposure on the proteome of peripheral blood mononuclear cells (PBMC) from the Asian catfish, Pangasianodon hypophthalmus. A common (0.1. ppm) concentration for therapeutic treatment was applied twice with a 72. h interval. PBMC were collected directly at the end of the second bath of MG (T1) and after 1. month of decontamination (T2). Analytical 2D-DIGE gels were run and a total of 2551 ± 364 spots were matched. Among them, MG induced significant changes in abundance of 116 spots with no recovery after one month of decontamination. Using LC-MS/MS and considering single identification per spot, we could identify 25 different proteins. Additionally, MG residues were measured in muscle and in blood indicating that leuco-MG has almost totally disappeared after one month of decontamination. This work highlights long term effects of MG treatment on the PBMC proteome from fish intended for human consumption.

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