This paper presents a very simple, industrially scalable method for transferring a high-resolution, biologically active protein pattern from one substrate to another. We demonstrate the transfer of a protein pattern formed initially by microcontact printing from a silicon surface (to which this form of printing is applicable) onto a glass or polymer substrate, almost independently of the surface/bulk properties of the second substrate. A very thin, spin-coated layer of a sugar is used to preserve the structure and organization of proteins during the subsequent plasma deposition of a siloxane polymer, after which the protein pattern could simply be peeled off the silicon substrate and glued onto any other desired substrate.
Volcke, C., Gandhiraman, R. P., Basabe-Desmonts, L., Iacono, M., Gubala, V., Cecchet, F., Cafolla, A. A., & Williams, D. E. (2010). Protein pattern transfer for biosensor applications. Biosensors and Bioelectronics, 25(6), 1295-1300. https://doi.org/10.1016/j.bios.2009.10.016